Overview
- External ontology
- mediadive.medium:J339 (source link unavailable) — SD0 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=339
Composition
flowchart LR
CultureMech_002698["sd0_medium"]
ing0_NaCl["NaCl"]
CultureMech_002698 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002698 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002698 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_NH4Cl["NH4Cl"]
CultureMech_002698 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002698 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002698 --> ing5_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
ing6_KH2PO4["KH2PO4"]
CultureMech_002698 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NaHCO3["NaHCO3"]
CultureMech_002698 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Yeast_extract["Yeast extract"]
CultureMech_002698 --> ing8_Yeast_extract
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002698 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002698 --> ing10_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002698 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002698 --> ing12_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002698 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002698 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002698 --> ing15_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing15_AlK_SO4_2 -.-> CHEBI_86463
ing16_H3BO3["H3BO3"]
CultureMech_002698 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002698 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Biotin["Biotin"]
CultureMech_002698 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_002698 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002698 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_002698 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_002698 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002698 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002698 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_002698 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002698 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Lipoic_acid["Lipoic acid"]
CultureMech_002698 --> ing27_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_Lipoic_acid -.-> CHEBI_16494
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
99.912 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 98.912, 1.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.412459999999999 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.41246, 3.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.96736 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.24728 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.238477 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.138477, 0.1] |
| 6 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.24728 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.138477 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.98912 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 | Yeast extract | 0.098912 G_PER_L | ||
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Adjust pH to 6.0 with HCl. Sterilize the medium by filtration.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sd0_medium (CultureMech:002698)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.335084Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J339 |
| 2026-03-13T03:09:06.189926Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:15.100019Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002698 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.447349+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SD0 MEDIUM" to "sd0_medium" for consistent matching |
| 2026-06-05T07:41:45.394239+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.340870+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.388709+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 12f46664123508d8f7ace65f6618db4b8f2d0817a22f1feedc0326ff93d68524 |