Overview
- External ontology
- mediadive.medium:J340 (source link unavailable) — MBM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=340
Composition
flowchart LR
CultureMech_002700["mbm_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_002700 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_KH2PO4["KH2PO4"]
CultureMech_002700 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_002700 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002700 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_002700 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002700 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002700 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Resazurin["Resazurin"]
CultureMech_002700 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_EDTA["EDTA"]
CultureMech_002700 --> ing8_EDTA
CHEBI_4735["CHEBI:4735"]
ing8_EDTA -.-> CHEBI_4735
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002700 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002700 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002700 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_002700 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002700 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002700 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002700 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002700 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.199601 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199601 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.199601 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.399202 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.199601 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0998004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.499002 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000998004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
[Merged 2 duplicates: 0.1, 0.1] |
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
[Merged 2 duplicates: 0.03, 0.03] |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.6 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.3, 0.3] |
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.4 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
[Merged 2 duplicates: 0.2, 0.2] |
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
[Merged 2 duplicates: 0.01, 0.01] |
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.04 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.02, 0.02] |
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.06 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.03, 0.03] |
Preparation
- Mix ingredients except SL-4 trace element solution and Na2S x 9H2O, and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Filter-sterilize SL-4 trace element solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add the Na2S x 9H2O solution to the medium, and adjust pH to 7.0, if necessary.
- Adjust pH to 3.6.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mbm_medium (CultureMech:002700)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.340056Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J340 |
| 2026-03-13T03:09:02.716692Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:19:15.113069Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002700 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.462886+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MBM MEDIUM" to "mbm_medium" for consistent matching |
| 2026-06-05T07:41:13.356159+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.043028+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.293456+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9edd9c34d054d8a90d841e200ff064b64060ea86c7b1efe2a5a635cd8fded51f |