thermococcus_medium_ph_6_0

CultureMech:002711 archaea COMPLEX LIQUID
Original name: THERMOCOCCUS MEDIUM (pH 6.0)

Overview

External ontology
mediadive.medium:J350 (source link unavailable) — THERMOCOCCUS MEDIUM (pH 6.0)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'thermococcus_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J280', 'original_category': 'archaea'}
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=350

Composition

flowchart LR
    CultureMech_002711["thermococcus_medium_ph_6_0"]
    ing0_KCl["KCl"]
    CultureMech_002711 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002711 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002711 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002711 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaCl["NaCl"]
    CultureMech_002711 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002711 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002711 --> ing6_Yeast_extract
    ing7_Tryptone["Tryptone"]
    CultureMech_002711 --> ing7_Tryptone
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002711 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_NaBr["NaBr"]
    CultureMech_002711 --> ing9_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing9_NaBr -.-> CHEBI_63004
    ing10_Sulfur["Sulfur"]
    CultureMech_002711 --> ing10_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing10_Sulfur -.-> CHEBI_26833
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002711 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Resazurin["Resazurin"]
    CultureMech_002711 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002711 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002711 --> ing14_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002711 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002711 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002711 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002711 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002711 --> ing19_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing19_AlK_SO4_2 -.-> CHEBI_86463
    ing20_H3BO3["H3BO3"]
    CultureMech_002711 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002711 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_Biotin["Biotin"]
    CultureMech_002711 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_Folic_acid["Folic acid"]
    CultureMech_002711 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002711 --> ing24_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing25_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002711 --> ing25_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing25_Thiamine_HCl -.-> CHEBI_49105
    ing26_Riboflavin["Riboflavin"]
    CultureMech_002711 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002711 --> ing27_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing27_Nicotinic_acid -.-> CHEBI_15940
    ing28_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002711 --> ing28_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing28_Calcium_pantothenate -.-> CHEBI_31345
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_002711 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_002711 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 KCl
Cl.K 74.551 g/mol
0.323529 G_PER_L CHEBI:32588
potassium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.7451 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.33333 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.33333, 3.0]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.245098 G_PER_L CHEBI:31206
ammonium chloride
5 NaCl
Cl.Na 58.443 g/mol
25.5098 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 24.5098, 1.0]
6 K2HPO4
HO4P.2K 174.174 g/mol
0.294118 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 Yeast extract 2.94118 G_PER_L
8 Tryptone 2.94118 G_PER_L
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1245098 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.0245098, 0.1]
10 NaBr
Br.Na 102.894 g/mol
0.00980392 G_PER_L CHEBI:63004
sodium bromide
11 Sulfur
S 32.067 g/mol
9.80392 G_PER_L CHEBI:26833
sulfur atom
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.490196 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Resazurin
C12H7NO4 229.191 g/mol
0.000980392 G_PER_L CHEBI:8806
Resazurin
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
24 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
25 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
26 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
28 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
29 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
30 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
32 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. After reduction of the medium, readjust pH to 6.0 with 1.0 N H2SO4.
  2. Mix ingredients except sulfur and Na2S x 9H2O, adjust pH to 7.0-7.2 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 7.0-7.2, if necessary.
  3. Comment:
  4. Most of <I>Thermococcus</I> strains will grow in this medium supplemented with 5.0 g/L maltodextrin and omitted sulfur.
  5. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:16.364429Z mediadive-import Imported from MediaDive Source: JCM, ID: J350
2026-03-13T03:09:10.058177Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:15.172037Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002711
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.531219+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOCOCCUS MEDIUM (pH 6.0)" to "thermococcus_medium_ph_6_0" for consistent matching
2026-06-05T07:41:49.937733+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:12.922930+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:20.963437+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: JCM_J280_THERMOCOCCUS_MEDIUM, thermococcus_medium_ph_6_0
2026-08-06T03:04:20.963495+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint ea91bad9e9faca8cb00b599932404ebf0ef8215502c85b975dc5674aed123ac9