modified_sulfate_reducing_bacteria_medium

Overview

External ontology

mediadive.medium:J354 (source link unavailable) — MODIFIED SULFATE REDUCING BACTERIA MEDIUM

Applications

• Microbial cultivation

pH

6.7

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=354

Composition

flowchart LR
    CultureMech_002714["modified_sulfate_reducing_bacteria_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002714 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002714 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002714 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002714 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_002714 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002714 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_PIPES["PIPES"]
    CultureMech_002714 --> ing6_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing6_PIPES -.-> CHEBI_39033
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002714 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Tryptone["Tryptone"]
    CultureMech_002714 --> ing8_Tryptone
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_002714 --> ing9_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate -.-> CHEBI_32954
    ing10_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002714 --> ing10_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing10_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing11_Na2SeO4["Na2SeO4"]
    CultureMech_002714 --> ing11_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing11_Na2SeO4 -.-> CHEBI_77775
    ing12_Vitamin_B12["Vitamin B12"]
    CultureMech_002714 --> ing12_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing12_Vitamin_B12 -.-> CHEBI_176843
    ing13_Resazurin["Resazurin"]
    CultureMech_002714 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002714 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Biotin["Biotin"]
    CultureMech_002714 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Folic_acid["Folic acid"]
    CultureMech_002714 --> ing16_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing16_Folic_acid -.-> CHEBI_27470
    ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002714 --> ing17_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing18_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002714 --> ing18_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing18_Thiamine_HCl -.-> CHEBI_49105
    ing19_Riboflavin["Riboflavin"]
    CultureMech_002714 --> ing19_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing19_Riboflavin -.-> CHEBI_17015
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002714 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002714 --> ing21_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing21_Calcium_pantothenate -.-> CHEBI_31345
    ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002714 --> ing22_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing23_Lipoic_acid["Lipoic acid"]
    CultureMech_002714 --> ing23_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing23_Lipoic_acid -.-> CHEBI_16494

Ingredients (24)

Preparation

1. Mix ingredients, except Na2S x 9H2O, and adjust pH to 6.7 with HCl. After autoclaving under a N2 atmosphere, distribute the medium into sterile culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Neutralize 5% Na2S x 9H2O solution, and autoclave under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium. Pressurize the inoculated vessels to 200 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• modified_sulfate_reducing_bacteria_medium (CultureMech:002714)