Overview
- External ontology
- mediadive.medium:J358 (source link unavailable) — ATYP MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.9
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=358
Composition
flowchart LR
CultureMech_002717["atyp_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002717 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002717 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002717 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002717 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_002717 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Sodium_acetate["Sodium acetate"]
CultureMech_002717 --> ing5_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Sodium_acetate -.-> CHEBI_32954
ing6_Sodium_succinate["Sodium succinate"]
CultureMech_002717 --> ing6_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_Sodium_succinate -.-> CHEBI_63675
ing7_Yeast_extract["Yeast extract"]
CultureMech_002717 --> ing7_Yeast_extract
ing8_Bacto_peptone["Bacto peptone"]
CultureMech_002717 --> ing8_Bacto_peptone
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002717 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002717 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002717 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002717 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002717 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_ZnCl2["ZnCl2"]
CultureMech_002717 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_H3BO3["H3BO3"]
CultureMech_002717 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002717 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002717 --> ing17_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing18_Biotin["Biotin"]
CultureMech_002717 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Niacin["Niacin"]
CultureMech_002717 --> ing19_Niacin
CHEBI_15940["CHEBI:15940"]
ing19_Niacin -.-> CHEBI_15940
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_002717 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002717 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002717 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002717 --> ing23_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_002717 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.998004 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.499002 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0998004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.998004 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.998004 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.998004 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.998004 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 8 | Yeast extract | 0.499002 G_PER_L | ||
| 9 | Bacto peptone | 0.299401 G_PER_L | ||
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.8 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.25 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.07 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.01 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Niacin
C6H5NO2 123.111 g/mol
|
0.35 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.3 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.2 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Adjust pH to 6.9.
- Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium.
- Adjust pH to 2 - 3 with HCl.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- atyp_medium (CultureMech:002717)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.388193Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J358 |
| 2026-03-13T06:19:15.232289Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002717 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.597399+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ATYP MEDIUM" to "atyp_medium" for consistent matching |
| 2026-06-05T07:40:50.072475+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.642185+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.443984+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b9f75144f6a32612fdf4c71f9a921f0f975f39b9f5610b90c4ee26dbffbded2f |