mmj_medium

CultureMech:002719 bacterial DEFINED LIQUID
Original name: MMJ MEDIUM

Overview

External ontology
mediadive.medium:J360 (source link unavailable) — MMJ MEDIUM
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=360

Composition

flowchart LR
    CultureMech_002719["mmj_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002719 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002719 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002719 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NaNO3["NaNO3"]
    CultureMech_002719 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002719 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002719 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_002719 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002719 --> ing7_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing8_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002719 --> ing8_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing8_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing9_Fe_SO4_3_x_n_H2O["Fe(SO4)3 x n H2O"]
    CultureMech_002719 --> ing9_Fe_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing9_Fe_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing10_H2WO4["H2WO4"]
    CultureMech_002719 --> ing10_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing10_H2WO4 -.-> CHEBI_36272
    ing11_NaHCO3["NaHCO3"]
    CultureMech_002719 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002719 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002719 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Resazurin["Resazurin"]
    CultureMech_002719 --> ing14_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Resazurin -.-> CHEBI_8806
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002719 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002719 --> ing16_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002719 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002719 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002719 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002719 --> ing20_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing20_AlK_SO4_2 -.-> CHEBI_86463
    ing21_H3BO3["H3BO3"]
    CultureMech_002719 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002719 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
29.703 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.990099 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NaNO3
NO3.Na 84.994 g/mol
0.792079 G_PER_L CHEBI:63005
sodium nitrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36634 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13861 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 KCl
Cl.K 74.551 g/mol
0.326733 G_PER_L CHEBI:32588
potassium chloride
8 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00495049 G_PER_L CHEBI:34887
nickel dichloride
9 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00495049 G_PER_L CHEBI:131361
disodium selenite pentahydrate
10 Fe(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
0.049505 G_PER_L CHEBI:53438
iron(3+) sulfate
11 H2WO4
H2O4W 249.852 g/mol
0.00495049 G_PER_L CHEBI:36272
tungstic acid
12 NaHCO3
CHO3.Na 84.006 g/mol
0.990099 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49505 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49505 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Resazurin
C12H7NO4 229.191 g/mol
0.000990099 G_PER_L CHEBI:8806
Resazurin
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.5 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.02 G_PER_L CHEBI:86463
potassium aluminium sulfate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients, except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, adjust pH to 7.5 - 8.0, and autoclave. Separately autoclave NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O solutions (5% each) under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.394047Z mediadive-import Imported from MediaDive Source: JCM, ID: J360
2026-03-13T06:19:15.245283Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002719
2026-03-17T06:30:33.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.615165+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJ MEDIUM" to "mmj_medium" for consistent matching
2026-06-05T07:41:35.487166+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.284744+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:47.201288+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 647890321030e3e907b170791e4f0679f60d7720c2e2b4b483598b4030fad2cd