deferribacter_abyssi_medium

Overview

External ontology

mediadive.medium:J365 (source link unavailable) — DEFERRIBACTER ABYSSI MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'caminibacter_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J367', 'original_category': 'bacterial'}

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=365

Composition

flowchart LR
    CultureMech_002724["deferribacter_abyssi_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002724 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KCl["KCl"]
    CultureMech_002724 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002724 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002724 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002724 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_NaCl["NaCl"]
    CultureMech_002724 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002724 --> ing6_Yeast_extract
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_002724 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Sulfur["Sulfur"]
    CultureMech_002724 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002724 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002724 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002724 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002724 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002724 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002724 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002724 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002724 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002724 --> ing17_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing17_AlK_SO4_2 -.-> CHEBI_86463
    ing18_H3BO3["H3BO3"]
    CultureMech_002724 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002724 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Biotin["Biotin"]
    CultureMech_002724 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_002724 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002724 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002724 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_002724 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002724 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002724 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_002724 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002724 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_002724 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494

Ingredients (30)

Preparation

1. Mix ingredients except CaCl2 x 2H2O, MgCl2 x 6H2O, sulfur and Na2S x 9H2O, adjust pH to 6.5 and autoclave. Separately autoclave CaCl2 x 2H2O and MgCl2 x 6H2O and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Autoclave neutralized 5% Na2S x 9H2O under a N2 atmosphere. Dispense the medium into culture vessels containing sulfur under a N2 atmosphere, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium and readjust pH, if necessary.
2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• caminibacter_medium (CultureMech:002726)
• deferribacter_abyssi_medium (CultureMech:002724)