mjais_medium

CultureMech:002727 bacterial DEFINED LIQUID
Original name: MJAIS MEDIUM

Overview

External ontology
mediadive.medium:J368 (source link unavailable) — MJAIS MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=368

Composition

flowchart LR
    CultureMech_002727["mjais_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002727 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002727 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002727 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002727 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002727 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002727 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002727 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_002727 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002727 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002727 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_002727 --> ing10_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing10_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing11_H2WO4["H2WO4"]
    CultureMech_002727 --> ing11_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing11_H2WO4 -.-> CHEBI_36272
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002727 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_Ferric_citrate["Ferric citrate"]
    CultureMech_002727 --> ing13_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing13_Ferric_citrate -.-> CHEBI_144421
    ing14_NaHCO3["NaHCO3"]
    CultureMech_002727 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139
    ing15_Sulfur["Sulfur"]
    CultureMech_002727 --> ing15_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing15_Sulfur -.-> CHEBI_26833
    ing16_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002727 --> ing16_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing16_Na2S_x_9_H2O -.-> CHEBI_76209
    ing17_Resazurin["Resazurin"]
    CultureMech_002727 --> ing17_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing17_Resazurin -.-> CHEBI_8806
    ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002727 --> ing18_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing19_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002727 --> ing19_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing19_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002727 --> ing20_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002727 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002727 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002727 --> ing23_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing23_AlK_SO4_2 -.-> CHEBI_86463
    ing24_H3BO3["H3BO3"]
    CultureMech_002727 --> ing24_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing24_H3BO3 -.-> CHEBI_33118
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002727 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002727 --> ing26_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing26_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing27_NaBr["NaBr"]
    CultureMech_002727 --> ing27_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing27_NaBr -.-> CHEBI_63004
    ing28_KI["KI"]
    CultureMech_002727 --> ing28_KI
    CHEBI_8346["CHEBI:8346"]
    ing28_KI -.-> CHEBI_8346
    ing29_Biotin["Biotin"]
    CultureMech_002727 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_002727 --> more["...9 more"]

Ingredients (39)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
29.6736 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.890208 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.692384 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.98912 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
1.2364 G_PER_L CHEBI:31206
ammonium chloride
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36301 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13452 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 KCl
Cl.K 74.551 g/mol
0.326409 G_PER_L CHEBI:32588
potassium chloride
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0049456 G_PER_L CHEBI:34887
nickel dichloride
10 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00049456 G_PER_L CHEBI:131361
disodium selenite pentahydrate
11 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
9.8912e-06 G_PER_L CHEBI:53438
iron(3+) sulfate
12 H2WO4
H2O4W 249.852 g/mol
0.0049456 G_PER_L CHEBI:36272
tungstic acid
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0197824 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 Ferric citrate
C6H5FeO7 244.944 g/mol
0.0197824 G_PER_L CHEBI:144421
iron(III) citrate
15 NaHCO3
CHO3.Na 84.006 g/mol
0.98912 G_PER_L CHEBI:32139
sodium hydrogencarbonate
16 Sulfur
S 32.067 g/mol
9.8912 G_PER_L CHEBI:26833
sulfur atom
17 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49456 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
18 Resazurin
C12H7NO4 229.191 g/mol
0.00098912 G_PER_L CHEBI:8806
Resazurin
19 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
20 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
21 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.5 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.02 G_PER_L CHEBI:86463
potassium aluminium sulfate
25 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.01 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
28 NaBr
Br.Na 102.894 g/mol
0.01 G_PER_L CHEBI:63004
sodium bromide
29 KI
I.K 166.002 g/mol
0.01 G_PER_L CHEBI:8346
potassium iodide
30 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
31 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
32 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
33 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
34 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
35 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
36 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
37 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
38 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
39 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.416660Z mediadive-import Imported from MediaDive Source: JCM, ID: J368
2026-03-13T06:19:15.303592Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002727
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.683409+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MJAIS MEDIUM" to "mjais_medium" for consistent matching
2026-06-05T07:41:35.078449+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 37 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.172271+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:46.741113+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 690f7b8c5656fada7711a220c7f13ae05821b5b80f419d613b1b7a30beebd37c