Overview
- External ontology
- mediadive.medium:J378 (source link unavailable) — ALKALIPHILUS CROTONATOXIDANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=378
Composition
flowchart LR
CultureMech_002735["alkaliphilus_crotonatoxidans_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002735 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002735 --> ing1_Tryptone
ing2_Crotonic_acid["Crotonic acid"]
CultureMech_002735 --> ing2_Crotonic_acid
CHEBI_41131["CHEBI:41131"]
ing2_Crotonic_acid -.-> CHEBI_41131
ing3_KH2PO4["KH2PO4"]
CultureMech_002735 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002735 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_NaCl["NaCl"]
CultureMech_002735 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NH4Cl["NH4Cl"]
CultureMech_002735 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002735 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_NaHCO3["NaHCO3"]
CultureMech_002735 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002735 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002735 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Resazurin["Resazurin"]
CultureMech_002735 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002735 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002735 --> ing13_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing13_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002735 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002735 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002735 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002735 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002735 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Na2SeO4["Na2SeO4"]
CultureMech_002735 --> ing19_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing19_Na2SeO4 -.-> CHEBI_77775
ing20_Na2WO4["Na2WO4"]
CultureMech_002735 --> ing20_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing20_Na2WO4 -.-> CHEBI_63940
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002735 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_Biotin["Biotin"]
CultureMech_002735 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_002735 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002735 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_002735 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_002735 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_002735 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002735 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_002735 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_002735 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 100 G_PER_L | ||
| 2 | Tryptone | 100 G_PER_L | ||
| 3 |
Crotonic acid
C4H6O2 86.09 g/mol
|
43 G_PER_L |
CHEBI:41131
crotonic acid
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
25 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
16.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
20 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
175 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.05 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.8 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.02 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 21 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.02 G_PER_L |
CHEBI:63940
sodium tungstate
|
|
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients, except crotonic acid, NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, in 900 ml of distilled water and adjust pH to 7.0-7.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense 0.9 volume of the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Add 0.1 volume of a filter-sterilized crotonic acid solution (0.86 g/100 ml, pH 7.0) to the medium. Autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the solutions of crotonic acid, L-cysteine x HCl x H2O and Na2S x 9H2O to the medium.
- Adjust pH to 6.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaliphilus_crotonatoxidans_medium (CultureMech:002735)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.447135Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J378 |
| 2026-01-28T19:48:14.962687Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:15.368718Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002735 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.757610+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILUS CROTONATOXIDANS MEDIUM" to "alkaliphilus_crotonatoxidans_medium" for consistent matching |
| 2026-06-05T07:40:48.085616+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.494615+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:52.866673+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.560588+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 05e4cdb704686c46d4facf6eb39f48c2778a23cce3c4e2687f68da7b81d70ae2 |