Overview
- External ontology
- mediadive.medium:J384 (source link unavailable) — GLYCEROL-SOIL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=384
Composition
flowchart LR
CultureMech_002741["glycerol_soil_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002741 --> ing0_Bacto_peptone
ing1_Beef_extract["Beef extract"]
CultureMech_002741 --> ing1_Beef_extract
ing2_Glycerol["Glycerol"]
CultureMech_002741 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_Soil_extract["Soil extract"]
CultureMech_002741 --> ing3_Soil_extract
ing4_Tap_water["Tap water"]
CultureMech_002741 --> ing4_Tap_water
CHEBI_15377["CHEBI:15377"]
ing4_Tap_water -.-> CHEBI_15377
ing5_Agar["Agar"]
CultureMech_002741 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 5 G_PER_L | ||
| 2 | Beef extract | 3 G_PER_L | ||
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
20 G_PER_L |
CHEBI:17754
glycerol
|
|
| 4 | Soil extract | 150 G_PER_L | ||
| 5 |
Tap water
H2O 18.015 g/mol
|
850 G_PER_L |
CHEBI:15377
water
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0.
- Suspend 400 g of air-dried garden soil in 960 ml of tap water and autoclave at 121C for 1 hr. After the mixture is cooled and settled, the supernatant is decanted, filtered through paper, autoclaved, and stored at room temperature until cleared by sedimentation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J384_GLYCEROL-SOIL_MEDIUM (CultureMech:002741)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.465655Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J384 |
| 2026-03-13T06:19:15.411150Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002741 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.805476+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLYCEROL-SOIL MEDIUM" to "glycerol_soil_medium" for consistent matching |
| 2026-06-05T07:40:17.191353+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:36.188998+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9c25667e7eed970ef00af2feaf40ee24449ceb0bc6ec59d36f0a9da3a4a98e33 |