methanosarcina_acetivorans_medium

CultureMech:002742 archaea COMPLEX LIQUID
Original name: METHANOSARCINA ACETIVORANS MEDIUM

Overview

External ontology
mediadive.medium:J385 (source link unavailable) — METHANOSARCINA ACETIVORANS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=385

Composition

flowchart LR
    CultureMech_002742["methanosarcina_acetivorans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002742 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002742 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002742 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KCl["KCl"]
    CultureMech_002742 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002742 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Na2HPO4["Na2HPO4"]
    CultureMech_002742 --> ing5_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4 -.-> CHEBI_34683
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002742 --> ing6_Yeast_extract
    ing7_Methanol["Methanol"]
    CultureMech_002742 --> ing7_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing7_Methanol -.-> CHEBI_17790
    ing8_Resazurin["Resazurin"]
    CultureMech_002742 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002742 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002742 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002742 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002742 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002742 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002742 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002742 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002742 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002742 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002742 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002742 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002742 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
23.1172 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 22.1172, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
11.93195 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 8.93195, 3.0]
3 NH4Cl
Cl.H4N 53.492 g/mol
0.94518 G_PER_L CHEBI:31206
ammonium chloride
4 KCl
Cl.K 74.551 g/mol
0.756144 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.232325 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.132325, 0.1]
6 Na2HPO4
HO4P.2Na 141.958 g/mol
0.567108 G_PER_L CHEBI:34683
disodium hydrogenphosphate
7 Yeast extract 0.94518 G_PER_L
8 Methanol
CH4O 32.042 g/mol
6.5 G_PER_L CHEBI:17790
methanol
9 Resazurin
C12H7NO4 229.191 g/mol
0.00094518 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components, except methanol, and bring to a boil for 5-10 seconds. Cool down under a stream of N2-CO2 (80:20, v/v), add methanol to the medium and adjust pH to 7.0 with HCl. Dispense the medium into suitable culture vessels (e. g. 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under an anaerobic gas atmosphere):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.467583Z mediadive-import Imported from MediaDive Source: JCM, ID: J385
2026-03-13T03:08:50.026611Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:15.419385Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002742
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.814189+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOSARCINA ACETIVORANS MEDIUM" to "methanosarcina_acetivorans_medium" for consistent matching
2026-06-05T07:41:32.339428+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.278807+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:31.101306+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 13f44a0eb1866e634d7a56ae6398bc4a3bd97f36caac7aadf09c3a207a0e2e70