Overview
- External ontology
- mediadive.medium:J399 (source link unavailable) — DESULFOVIBRIO MAGNETICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=399
Composition
flowchart LR
CultureMech_002755["desulfovibrio_magneticus_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002755 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NH4Cl["NH4Cl"]
CultureMech_002755 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Sodium_fumarate["Sodium fumarate"]
CultureMech_002755 --> ing2_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing2_Sodium_fumarate -.-> CHEBI_115156
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002755 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002755 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002755 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_Quinic_acid["Quinic acid"]
CultureMech_002755 --> ing6_Quinic_acid
CHEBI_26493["CHEBI:26493"]
ing6_Quinic_acid -.-> CHEBI_26493
ing7_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002755 --> ing7_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing7_Nitrilotriacetic_acid -.-> CHEBI_44557
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002755 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002755 --> ing9_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
ing10_NaCl["NaCl"]
CultureMech_002755 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002755 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002755 --> ing12_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002755 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002755 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002755 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002755 --> ing16_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing16_AlK_SO4_2 -.-> CHEBI_86463
ing17_H3BO3["H3BO3"]
CultureMech_002755 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002755 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Biotin["Biotin"]
CultureMech_002755 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_002755 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002755 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_002755 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_002755 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_002755 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002755 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_002755 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002755 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_002755 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.197239 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0591716 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
0.571992 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.433925 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.0493097 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
4.5 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 7 | Quinic acid | 1.9 G_PER_L |
CHEBI:26493
quinic acid
|
|
| 8 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfovibrio_magneticus_medium (CultureMech:002755)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.506189Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J399 |
| 2026-03-13T06:19:15.502274Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002755 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.908260+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIBRIO MAGNETICUS MEDIUM" to "desulfovibrio_magneticus_medium" for consistent matching |
| 2026-06-05T07:40:57.831626+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.045937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:32.232959+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d89af152f05851746708d92b79c82aa887872adc18ed720fac25af6028d9d0cf |