Overview
- External ontology
- mediadive.medium:J405 (source link unavailable) — MODIFIED HNW MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=405
Composition
flowchart LR
CultureMech_002761["modified_hnw_medium"]
ing0_Sodium_dithionite["Sodium dithionite"]
CultureMech_002761 --> ing0_Sodium_dithionite
CHEBI_66870["CHEBI:66870"]
ing0_Sodium_dithionite -.-> CHEBI_66870
ing1_NH4Cl["NH4Cl"]
CultureMech_002761 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaNO3["NaNO3"]
CultureMech_002761 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002761 --> ing3_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing3_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing4_NaHCO3["NaHCO3"]
CultureMech_002761 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_NaCl["NaCl"]
CultureMech_002761 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_K2HPO4["K2HPO4"]
CultureMech_002761 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002761 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002761 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002761 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_KCl["KCl"]
CultureMech_002761 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002761 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002761 --> ing12_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing13_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002761 --> ing13_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing13_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing14_Biotin["Biotin"]
CultureMech_002761 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_002761 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002761 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl["Thiamine HCl"]
CultureMech_002761 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Riboflavin["Riboflavin"]
CultureMech_002761 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_002761 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002761 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_002761 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002761 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Lipoic_acid["Lipoic acid"]
CultureMech_002761 --> ing23_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23_Lipoic_acid -.-> CHEBI_16494
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium dithionite
2Na.O4S2 174.11 g/mol
|
25 G_PER_L |
CHEBI:66870
sodium dithionite
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.244618 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
0.978474 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 4 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
9.78474e-05 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
12.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
29.703 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.138614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 14 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Use Medium No. 344. Prior to inoculation, add per liter the following solution (freshly prepared and filter-sterilized):
- Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
- Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized):
- Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
- Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_hnw_medium (CultureMech:002761)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.525593Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J405 |
| 2026-01-28T19:48:26.205239Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:15.554221Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002761 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.966645+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED HNW MEDIUM" to "modified_hnw_medium" for consistent matching |
| 2026-06-05T07:41:36.740099+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.665588+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.432980+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 211495a083ade13118e06f21e8ef6b7685b31f75bcd2d64844467c7ec0a06b49 |