Overview
- External ontology
- mediadive.medium:J408 (source link unavailable) — 15% MH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=408
Composition
flowchart LR
CultureMech_002764["15_mh_medium"]
ing0_NaCl["NaCl"]
CultureMech_002764 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002764 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002764 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002764 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_002764 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_002764 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_002764 --> ing6_Proteose_peptone_no__3
ing7_Yeast_extract["Yeast extract"]
CultureMech_002764 --> ing7_Yeast_extract
ing8_Glucose["Glucose"]
CultureMech_002764 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Agar["Agar"]
CultureMech_002764 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
121.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
14.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.54 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.09 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | Proteose peptone no. 3 | 5 G_PER_L | ||
| 8 | Yeast extract | 10 G_PER_L | ||
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J408_15_MH_MEDIUM (CultureMech:002764)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.532712Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J408 |
| 2026-03-13T06:19:15.566981Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002764 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.981359+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "15% MH MEDIUM" to "15_mh_medium" for consistent matching |
| 2026-06-05T07:40:17.213187+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:36.214137+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b586cb857b2f3febbe6c3c513ea0567c1ef25bf0a0f159a0efeced0292e9039d |