Overview
- External ontology
- mediadive.medium:J429 (source link unavailable) — NPB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=429
Composition
flowchart LR
CultureMech_002781["npb_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_002781 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002781 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002781 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2HPO4["K2HPO4"]
CultureMech_002781 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_002781 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_D_Glucose["D-Glucose"]
CultureMech_002781 --> ing5_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing5_D_Glucose -.-> CHEBI_17634
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J429_NPB_MEDIUM (CultureMech:002781)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.590178Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J429 |
| 2026-03-13T06:19:15.670670Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002781 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.098368+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NPB MEDIUM" to "npb_medium" for consistent matching |
| 2026-06-05T07:40:17.255347+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.966042+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:36.301053+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dbe031706eb4274fc52f8c1f1887263ad36af095219b9caaf4efa3477a8ec569 |