Overview
- External ontology
- mediadive.medium:J458 (source link unavailable) — B MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.7
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=458
Composition
flowchart LR
CultureMech_002807["b_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002807 --> ing0_Bacto_peptone
ing1_Casamino_acids["Casamino acids"]
CultureMech_002807 --> ing1_Casamino_acids
ing2_Yeast_extract["Yeast extract"]
CultureMech_002807 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002807 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_KH2PO4["KH2PO4"]
CultureMech_002807 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002807 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Agar["Agar"]
CultureMech_002807 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Sea_water["Sea water"]
CultureMech_002807 --> ing7_Sea_water
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 2 G_PER_L | ||
| 2 | Casamino acids | 2 G_PER_L | ||
| 3 | Yeast extract | 2 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 8 | Sea water | 500 G_PER_L |
Preparation
- Adjust pH to 7.5 - 7.8.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- b_medium (CultureMech:002807)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.667318Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J458 |
| 2026-01-28T19:48:25.591128Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:15.847642Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002807 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.298730+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "B MEDIUM" to "b_medium" for consistent matching |
| 2026-06-05T07:40:50.204367+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.940392+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:53.691302+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.541361+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5bd655f7040baf7c163b1d4e762eaf3a8a363d23fd4eef6fa70799a75aab643b |