saltwater_medium_with_lactate

Overview

External ontology

mediadive.medium:J462 (source link unavailable) — SALTWATER MEDIUM WITH LACTATE

Applications

• Microbial cultivation

Synonyms

• {'name': 'saltwater_medium_with_acetate_and_ferric_citrate', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J463', 'original_category': 'bacterial'}

pH

7.0

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=462

Composition

flowchart LR
    CultureMech_002811["saltwater_medium_with_lactate"]
    ing0_NaCl["NaCl"]
    CultureMech_002811 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2SO4["Na2SO4"]
    CultureMech_002811 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002811 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002811 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002811 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002811 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_KBr["KBr"]
    CultureMech_002811 --> ing6_KBr
    CHEBI_32030["CHEBI:32030"]
    ing6_KBr -.-> CHEBI_32030
    ing7_KCl["KCl"]
    CultureMech_002811 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002811 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_002811 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002811 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Resazurin["Resazurin"]
    CultureMech_002811 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_Riboflavin["Riboflavin"]
    CultureMech_002811 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_HCl["HCl"]
    CultureMech_002811 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002811 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_H3BO3["H3BO3"]
    CultureMech_002811 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002811 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002811 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002811 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002811 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002811 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002811 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002811 --> ing22_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing23_Biotin["Biotin"]
    CultureMech_002811 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002811 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002811 --> ing25_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002811 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Sodium_phosphate_buffer["Sodium phosphate buffer"]
    CultureMech_002811 --> ing27_Sodium_phosphate_buffer
    ing28_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002811 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_002811 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_002811 --> more["...3 more"]

Ingredients (33)

Preparation

1. Dissolve components except NaHCO3, Vitamin solution, Thiamine solution, Vitamin B12 solution, sodium lactate and Na2S x 9H2O in 900 ml distilled water and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution, and filter-sterilized 8% NaHCO3 solution, Vitamin solution, Thiamine solution and Vitamin B12 solution to the medium. Readjust pH to 7.0, if necessary. Prior to use, reduce the medium with Na2S x 9H2O (3% solution, autoclaved and stocked under N2).

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• saltwater_medium_with_acetate_and_ferric_citrate (CultureMech:002812)
• saltwater_medium_with_lactate (CultureMech:002811)