Overview
- External ontology
- mediadive.medium:J480 (source link unavailable) — MODIFIED SPOROMUSA MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=480
Composition
flowchart LR
CultureMech_002830["modified_sporomusa_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002830 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002830 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_002830 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002830 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002830 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NaCl["NaCl"]
CultureMech_002830 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NaHCO3["NaHCO3"]
CultureMech_002830 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Resazurin["Resazurin"]
CultureMech_002830 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Dithiothreitol["Dithiothreitol"]
CultureMech_002830 --> ing8_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing8_Dithiothreitol -.-> CHEBI_18320
ing9_Mannitol["Mannitol"]
CultureMech_002830 --> ing9_Mannitol
CHEBI_29864["CHEBI:29864"]
ing9_Mannitol -.-> CHEBI_29864
ing10_Yeast_extract["Yeast extract"]
CultureMech_002830 --> ing10_Yeast_extract
ing11_Casitone["Casitone"]
CultureMech_002830 --> ing11_Casitone
ing12_Biotin["Biotin"]
CultureMech_002830 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_002830 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002830 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_002830 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Riboflavin["Riboflavin"]
CultureMech_002830 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_002830 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002830 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_002830 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002830 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Lipoic_acid["Lipoic acid"]
CultureMech_002830 --> ing21_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21_Lipoic_acid -.-> CHEBI_16494
ing22_Na2SeO3["Na2SeO3"]
CultureMech_002830 --> ing22_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing22_Na2SeO3 -.-> CHEBI_48843
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002830 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
29 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
18.9167 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
41.6667 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
41.6667 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
20.8333 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
187.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
333.333 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0833333 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
25 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 10 |
Mannitol
C6H14O6 182.172 g/mol
|
300 G_PER_L |
CHEBI:29864
mannitol
|
|
| 11 | Yeast extract | 41.6667 G_PER_L | ||
| 12 | Casitone | 41.6667 G_PER_L | ||
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 23 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.004 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve components, except NaHCO3, dithiothreitol and mannitol, in 900 ml distilled water and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately autoclave 3% (w/v) dithiothreitol solution and 1 M mannitol solution under a N2 atmosphere. Add filter-sterilized 5% NaHCO3 [stored under a N2-CO2 (4:1, v/v) atmosphere] to the medium. Prior to inoculation, add the sterile dithiothreitol and mannitol solutions.
- Use Trace element solution of Medium No. 243 without Na2SeO3 x 5H2O.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_sporomusa_medium (CultureMech:002830)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.712708Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J480 |
| 2026-03-13T06:19:15.960526Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002830 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.427591+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED SPOROMUSA MEDIUM" to "modified_sporomusa_medium" for consistent matching |
| 2026-06-05T07:41:37.790873+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.955660+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.830816+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4939c205b168bd91428ad689af23c3bc3b1b687d0cd9798dd92a7f176d512d4e |