Overview
- External ontology
- mediadive.medium:J483 (source link unavailable) — ETHANOLOGENBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=483
Composition
flowchart LR
CultureMech_002833["ethanologenbacterium_medium"]
ing0_Glucose["Glucose"]
CultureMech_002833 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Tryptone["Tryptone"]
CultureMech_002833 --> ing1_Tryptone
ing2_Beef_extract["Beef extract"]
CultureMech_002833 --> ing2_Beef_extract
ing3_Yeast_extract["Yeast extract"]
CultureMech_002833 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_002833 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_K2HPO4["K2HPO4"]
CultureMech_002833 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002833 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002833 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002833 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002833 --> ing9_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002833 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_H3BO3["H3BO3"]
CultureMech_002833 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002833 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002833 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002833 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002833 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002833 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_Cyanocobalamin["Cyanocobalamin"]
CultureMech_002833 --> ing17_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing17_Cyanocobalamin -.-> CHEBI_17439
ing18_Ascorbic_acid["Ascorbic acid"]
CultureMech_002833 --> ing18_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing18_Ascorbic_acid -.-> CHEBI_22652
ing19_Riboflavin["Riboflavin"]
CultureMech_002833 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Citric_acid["Citric acid"]
CultureMech_002833 --> ing20_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing20_Citric_acid -.-> CHEBI_30769
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002833 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Folic_acid["Folic acid"]
CultureMech_002833 --> ing22_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing22_Folic_acid -.-> CHEBI_27470
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002833 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Creatine["Creatine"]
CultureMech_002833 --> ing24_Creatine
CHEBI_16919["CHEBI:16919"]
ing24_Creatine -.-> CHEBI_16919
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
9.80392 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Tryptone | 3.92157 G_PER_L | ||
| 3 | Beef extract | 1.96078 G_PER_L | ||
| 4 | Yeast extract | 0.980392 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
3.92157 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.47059 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0980392 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.196078 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.490196 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.01 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.05 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
4.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.01 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 19 | Ascorbic acid | 0.025 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.025 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Citric acid
C6H8O7 192.123 g/mol
|
0.02 G_PER_L |
CHEBI:30769
citric acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.05 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.01 G_PER_L |
CHEBI:27470
folic acid
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
Creatine
C4H9N3O2 131.135 g/mol
|
0.025 G_PER_L |
CHEBI:16919
creatine
|
Preparation
- Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ethanologenbacterium_medium (CultureMech:002833)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.719028Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J483 |
| 2026-03-13T06:19:15.975523Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002833 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.445779+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ETHANOLOGENBACTERIUM MEDIUM" to "ethanologenbacterium_medium" for consistent matching |
| 2026-06-05T07:40:59.686531+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.737399+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.701814+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 27eb2844cd68347f41b037e2d8e215e984c8d674b054783b82a343f029334add |