Overview
- External ontology
- mediadive.medium:J492 (source link unavailable) — MMJ-TL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=492
Composition
flowchart LR
CultureMech_002843["mmj_tl_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_002843 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Sulfur["Sulfur"]
CultureMech_002843 --> ing1_Sulfur
CHEBI_26833["CHEBI:26833"]
ing1_Sulfur -.-> CHEBI_26833
ing2_Resazurin["Resazurin"]
CultureMech_002843 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002843 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_NaCl["NaCl"]
CultureMech_002843 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_K2HPO4["K2HPO4"]
CultureMech_002843 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002843 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NH4Cl["NH4Cl"]
CultureMech_002843 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002843 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002843 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_KCl["KCl"]
CultureMech_002843 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002843 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002843 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002843 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing14_Biotin["Biotin"]
CultureMech_002843 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_002843 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002843 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl["Thiamine HCl"]
CultureMech_002843 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Riboflavin["Riboflavin"]
CultureMech_002843 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_002843 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002843 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_002843 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002843 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Lipoic_acid["Lipoic acid"]
CultureMech_002843 --> ing23_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23_Lipoic_acid -.-> CHEBI_16494
ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002843 --> ing24_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
ing25_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002843 --> ing25_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing25_MnSO4_x_n_H2O -.-> CHEBI_86360
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002843 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002843 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002843 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002843 --> ing29_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing29_AlK_SO4_2 -.-> CHEBI_86463
CultureMech_002843 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.990099 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
Sulfur
S 32.067 g/mol
|
2.9703 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
31.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 30.0, 1.0] |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.5, 0.1] |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.4, 3.0] |
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.11 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.01, 0.1] |
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.001 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.001 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 25 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 26 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 31 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 32 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix ingredients except NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 7.0 with NaOH and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add 5% Na2S x 9H2O (autoclaved and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmj_tl_medium (CultureMech:002843)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.739600Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J492 |
| 2026-03-13T03:08:37.440537Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:16.027194Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002843 |
| 2026-03-17T06:29:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.506160+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-TL MEDIUM" to "mmj_tl_medium" for consistent matching |
| 2026-06-05T07:41:35.548044+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.303745+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.263700+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 16b3949cd62d698443e7b6f79e5ab711cf7dac86f7c1c9e4f6de4fbbb6394070 |