Overview
- External ontology
- mediadive.medium:J512 (source link unavailable) — A1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=512
Composition
flowchart LR
CultureMech_002863["a1_medium"]
ing0_NaCl["NaCl"]
CultureMech_002863 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_002863 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_NH4Cl["NH4Cl"]
CultureMech_002863 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KH2PO4["KH2PO4"]
CultureMech_002863 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002863 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_NaHCO3["NaHCO3"]
CultureMech_002863 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002863 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002863 --> ing7_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4_x_n_H2O -.-> CHEBI_86360
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002863 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002863 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002863 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_ZnCl2["ZnCl2"]
CultureMech_002863 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002863 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002863 --> ing13_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing14_H3BO3["H3BO3"]
CultureMech_002863 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002863 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002863 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002863 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_Biotin["Biotin"]
CultureMech_002863 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Pantothenic_acid["Pantothenic acid"]
CultureMech_002863 --> ing19_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing19_Pantothenic_acid -.-> CHEBI_7916
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002863 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002863 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Thioctic_acid["Thioctic acid"]
CultureMech_002863 --> ing22_Thioctic_acid
CHEBI_30314["CHEBI:30314"]
ing22_Thioctic_acid -.-> CHEBI_30314
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002863 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_002863 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_002863 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002863 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_002863 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002863 --> ing28_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing28_MgCl2_x_6_H2O -.-> CHEBI_86345
ing29_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002863 --> ing29_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing29_Na2S_x_9_H2O -.-> CHEBI_76209
CultureMech_002863 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20.6078 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 19.6078, 1.0] |
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.754902 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.245098 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0980392 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.196078 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.196078, 3.0] |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.96078 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
( trisodium salt) |
| 8 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
30.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.1, 30.4] |
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.13 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.025 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.005 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 21 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 29 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
212 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 30 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
40 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 31 |
Galactose
C6H12O6 180.156 g/mol
|
90.1 G_PER_L |
CHEBI:28260
galactose
|
Preparation
- Mix ingredients except NaHCO3, adjust pH to 6.8 and add NaHCO3 to the medium. Dispense the medium (9 ml) into 26-ml anaerobic pressure tubes under a stream of oxygen-free N2-CO2 (80:20) and seal. After autoclaving, aseptically and anaerobically add 0.2 ml APM salts solution and 0.1 ml Sulfide stock solution and 0.1 ml Galactose stock solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J512_A1_MEDIUM (CultureMech:002863)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.800647Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J512 |
| 2026-03-13T03:08:59.008390Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:16.165328Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002863 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.674685+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A1 MEDIUM" to "a1_medium" for consistent matching |
| 2026-06-05T07:40:17.430060+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.019143+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:36.909921+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a219e35bb8a37961c1c4008e2616562649210bca04fad77aca71633bd4b56145 |