modified_desulfoglaeba_alkanexedens_medium

CultureMech:002872 bacterial COMPLEX LIQUID
Original name: MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM

Overview

External ontology
mediadive.medium:J521 (source link unavailable) — MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=521

Composition

flowchart LR
    CultureMech_002872["modified_desulfoglaeba_alkanexedens_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_002872 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002872 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002872 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_Resazurin["Resazurin"]
    CultureMech_002872 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_NaHCO3["NaHCO3"]
    CultureMech_002872 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002872 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_002872 --> ing6_Trace_vitamins__see_Medium_No__197_
    ing7_Decane["Decane"]
    CultureMech_002872 --> ing7_Decane
    CHEBI_41808["CHEBI:41808"]
    ing7_Decane -.-> CHEBI_41808
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002872 --> ing8_Yeast_extract
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002872 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002872 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Sea Salt 27.7469 G_PER_L
2 NH4Cl
Cl.H4N 53.492 g/mol
0.277469 G_PER_L CHEBI:31206
ammonium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
3.88457 G_PER_L CHEBI:32149
sodium sulfate
4 Resazurin
C12H7NO4 229.191 g/mol
0.00110988 G_PER_L CHEBI:8806
Resazurin
5 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.5 G_PER_L CHEBI:50144
sodium pyruvate
7 Trace vitamins (see Medium No. 197) 0.1 G_PER_L
8 Decane
C10H22 142.286 g/mol
0.01 G_PER_L CHEBI:41808
decane
9 Yeast extract 0.05 G_PER_L
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
12.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
12.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Mix components and adjust pH to 7.2 - 7.4. Boil Mineral medium and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute Mineral medium into culture vessels (e.g., 9.0 ml of the medium in tubes) under the same gas mixture, seal with Teflon-coated rubber stoppers and autoclave. After cooling, add the following components (per 9.0 ml Mineral medium) from sterile anoxic stock solutions (autoclaved or *filter-sterilized):
  2. Autoclave and store under a N2 atmosphere.
  3. Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.828744Z mediadive-import Imported from MediaDive Source: JCM, ID: J521
2026-03-13T06:19:16.237937Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002872
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.755762+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED DESULFOGLAEBA ALKANEXEDENS MEDIUM" to "modified_desulfoglaeba_alkanexedens_medium" for consistent matching
2026-06-05T07:41:36.521087+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.624440+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:48.221343+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f10b64fd3177e8acc1ab4683d2def963cafd0f1ae0f911120e85adc99766ba27