Overview
- External ontology
- mediadive.medium:J536 (source link unavailable) — PHE91 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=536
Composition
flowchart LR
CultureMech_002884["phe91_medium"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002884 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_K2HPO4["K2HPO4"]
CultureMech_002884 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Resazurin["Resazurin"]
CultureMech_002884 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Dithionite["Dithionite"]
CultureMech_002884 --> ing3_Dithionite
CHEBI_42160["CHEBI:42160"]
ing3_Dithionite -.-> CHEBI_42160
ing4_Pyruvate["Pyruvate"]
CultureMech_002884 --> ing4_Pyruvate
CHEBI_15361["CHEBI:15361"]
ing4_Pyruvate -.-> CHEBI_15361
ing5_NH4H2PO4["NH4H2PO4"]
CultureMech_002884 --> ing5_NH4H2PO4
CHEBI_62982["CHEBI:62982"]
ing5_NH4H2PO4 -.-> CHEBI_62982
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002884 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_HCl["HCl"]
CultureMech_002884 --> ing7_HCl
CHEBI_17883["CHEBI:17883"]
ing7_HCl -.-> CHEBI_17883
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002884 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002884 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002884 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_ZnCl2["ZnCl2"]
CultureMech_002884 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_H3BO3["H3BO3"]
CultureMech_002884 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002884 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002884 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002884 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_Biotin["Biotin"]
CultureMech_002884 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_002884 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_002884 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002884 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002884 --> ing20_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_DL_Calcium_pantothenate -.-> CHEBI_31345
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002884 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_002884 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_NaOH["NaOH"]
CultureMech_002884 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002884 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002884 --> ing25_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Dithionite
O4S2 128.13 g/mol
|
5 G_PER_L |
CHEBI:42160
dithionite(2-)
|
|
| 5 |
Pyruvate
C3H3O3 87.054 g/mol
|
10 G_PER_L |
CHEBI:15361
pyruvate
|
|
| 6 |
NH4H2PO4
H2O4P.H4N 115.025 g/mol
|
0.474716 G_PER_L |
CHEBI:62982
ammonium dihydrogen phosphate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.505676 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
HCl
HCl 36.461 g/mol
|
10 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.48515 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.188119 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0990099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0693069 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.0613861 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0356436 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0237624 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0168317 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0025 G_PER_L |
CHEBI:15956
biotin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0125 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0125 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0065 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0625 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 26 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Autoclave the basal solution under a N2 gas atmosphere. Separately filter-sterilize the other solutions and add to the medium anaerobically and aseptically. Prepare dithionite solution just before use.
- Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components.
- Store in the dark at 4C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- phe91_medium (CultureMech:002884)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.863888Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J536 |
| 2026-03-13T06:19:16.314278Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002884 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.848044+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PHE91 MEDIUM" to "phe91_medium" for consistent matching |
| 2026-06-05T07:41:41.752580+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.180207+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.871699+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bf798fea0023e788992d83fdcee9e3eb174ea30321efb405cc34ab58f340952f |