Overview
- External ontology
- mediadive.medium:J538 (source link unavailable) — BRUCELLA BROTH
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=538
Composition
flowchart LR
CultureMech_002886["brucella_broth"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_002886 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_animal_tissue["Peptic digest of animal tissue"]
CultureMech_002886 --> ing1_Peptic_digest_of_animal_tissue
ing2_Glucose["Glucose"]
CultureMech_002886 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Yeast_extract["Yeast extract"]
CultureMech_002886 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_002886 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_NaHSO3["NaHSO3"]
CultureMech_002886 --> ing5_NaHSO3
CHEBI_26709["CHEBI:26709"]
ing5_NaHSO3 -.-> CHEBI_26709
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Pancreatic digest of casein | 10 G_PER_L | ||
| 2 | Peptic digest of animal tissue | 10 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Yeast extract | 2 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
NaHSO3
HO3S.Na 104.062 g/mol
|
0.1 G_PER_L |
CHEBI:26709
sodium hydrogensulfite
|
Preparation
- Adjust pH to 7.0.
- Comment: Commercial Brucella broth (Becton & Dickinson or Oxoid) is also available.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- brucella_broth (CultureMech:002886)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.873633Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J538 |
| 2026-03-13T06:19:16.337060Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002886 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.875593+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BRUCELLA BROTH" to "brucella_broth" for consistent matching |
| 2026-06-05T07:40:51.586134+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:26.005048+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a969823cd9d40a372ce1506f81a452d18854983101511c491745e5d21e8447c2 |