Overview
- External ontology
- mediadive.medium:J547 (source link unavailable) — DESULFOTOMACULUM PBE MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=547
Composition
flowchart LR
CultureMech_002894["desulfotomaculum_pbe_medium"]
ing0_Peptone["Peptone"]
CultureMech_002894 --> ing0_Peptone
ing1_Beef_extract["Beef extract"]
CultureMech_002894 --> ing1_Beef_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002894 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Na2SO4["Na2SO4"]
CultureMech_002894 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_CaCl2["CaCl2"]
CultureMech_002894 --> ing4_CaCl2
CHEBI_3312["CHEBI:3312"]
ing4_CaCl2 -.-> CHEBI_3312
ing5_K2HPO4["K2HPO4"]
CultureMech_002894 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Sodium_lactate["Sodium lactate"]
CultureMech_002894 --> ing6_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing6_Sodium_lactate -.-> CHEBI_75228
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002894 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_Sodium_ascorbate["Sodium ascorbate"]
CultureMech_002894 --> ing8_Sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing8_Sodium_ascorbate -.-> CHEBI_113451
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 2.0202 G_PER_L | ||
| 2 | Beef extract | 1.0101 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.0202 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.51515 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.10101 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
70 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
10 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 |
Sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
10 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Preparation
- Mix components and autoclave under a N2 gas atmosphere.
- To complete the medium, aseptically and anaerobically add the following solutions to the medium:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfotomaculum_pbe_medium (CultureMech:002894)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.901288Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J547 |
| 2026-01-28T19:48:38.997728Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:16.403641Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002894 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.950373+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOTOMACULUM PBE MEDIUM" to "desulfotomaculum_pbe_medium" for consistent matching |
| 2026-06-05T07:40:57.584627+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.952860+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.999958+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4b51f416b03225d4192519a7ddf1dd2c4306ce095cbe97f2bbb9244bc01d342f |