Overview
- External ontology
- mediadive.medium:J548 (source link unavailable) — GEOSPOROBACTER SUBTERRENEUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=548
Composition
flowchart LR
CultureMech_002895["geosporobacter_subterreneus_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_002895 --> ing0_Sea_Salt
ing1_NH4Cl["NH4Cl"]
CultureMech_002895 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Resazurin["Resazurin"]
CultureMech_002895 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Yeast_extract["Yeast extract"]
CultureMech_002895 --> ing3_Yeast_extract
ing4_Glucose["Glucose"]
CultureMech_002895 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_NaHCO3["NaHCO3"]
CultureMech_002895 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002895 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 10.8696 G_PER_L | ||
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.08696 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00108696 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 | Yeast extract | 0.05 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.05 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Mix ingredients and adjust pH to 7.2. Distribute 0.92 volume of the medium into tubes (e.g., 4.6 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
- To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.6 ml):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- geosporobacter_subterreneus_medium (CultureMech:002895)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.903537Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J548 |
| 2026-03-13T06:19:16.408334Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002895 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.955798+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEOSPOROBACTER SUBTERRENEUS MEDIUM" to "geosporobacter_subterreneus_medium" for consistent matching |
| 2026-06-05T07:41:06.291030+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.081370+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.155688+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5a22a57c3da38df553aa23fc0c36595fcb7ea098297266964dfbc0af2b9aa6b2 |