Overview
- External ontology
- mediadive.medium:J550 (source link unavailable) — AMINIPHILUS CIRCUMSRIPTUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=550
Composition
flowchart LR
CultureMech_002898["aminiphilus_circumsriptus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002898 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002898 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002898 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_002898 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_002898 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2["CaCl2"]
CultureMech_002898 --> ing5_CaCl2
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2 -.-> CHEBI_3312
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002898 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Yeast_extract["Yeast extract"]
CultureMech_002898 --> ing7_Yeast_extract
ing8_Peptone["Peptone"]
CultureMech_002898 --> ing8_Peptone
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002898 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002898 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002898 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002898 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002898 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002898 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002898 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002898 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002898 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002898 --> ing18_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing18_AlK_SO4_2 -.-> CHEBI_86463
ing19_H3BO3["H3BO3"]
CultureMech_002898 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002898 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.29703 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.29703 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.990099 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
10.90099 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 9.90099, 1.0] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.0990099 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.0990099 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.49505 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 | Yeast extract | 1.9802 G_PER_L | ||
| 9 | Peptone | 9.90099 G_PER_L | ||
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49505 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix ingredients and adjust pH to 7.0. Distribute into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- aminiphilus_circumsriptus_medium (CultureMech:002898)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.909312Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J550 |
| 2026-03-13T03:08:53.232912Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:16.429346Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002898 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.979231+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AMINIPHILUS CIRCUMSRIPTUS MEDIUM" to "aminiphilus_circumsriptus_medium" for consistent matching |
| 2026-06-05T07:40:48.417448+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.999514+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.903087+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 551f5ee40fbe8498e8263949747e7ea3cb988117bdf6e7311723061d28a4c1b9 |