Overview
- External ontology
- mediadive.medium:J557 (source link unavailable) — ALKALINE LB AGAR
- Applications
-
- Microbial cultivation
- pH
- 8.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=557
Composition
flowchart LR
CultureMech_002905["alkaline_lb_agar"]
ing0_Tryptone["Tryptone"]
CultureMech_002905 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002905 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002905 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Na2CO3["Na2CO3"]
CultureMech_002905 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Agar["Agar"]
CultureMech_002905 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Separately sterilize Na2CO3 by filtration and aseptically add to the medium. Check final pH to be about 8.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaline_lb_agar (CultureMech:002905)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.927915Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J557 |
| 2026-03-13T06:19:16.471126Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002905 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.026957+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE LB AGAR" to "alkaline_lb_agar" for consistent matching |
| 2026-06-05T07:40:47.886869+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.803288+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.387888+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b6acb1cd1c7ab5eb43edf4c4a5c258a1d67a1dd18027c6a6b241fa48656f7f9b |