magnetospirillum_medium_a

CultureMech:002906 bacterial DEFINED LIQUID
Original name: MAGNETOSPIRILLUM MEDIUM (A)

Overview

External ontology
mediadive.medium:J558 (source link unavailable) — MAGNETOSPIRILLUM MEDIUM (A)
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=558

Composition

flowchart LR
    CultureMech_002906["magnetospirillum_medium_a"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_002906 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002906 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002906 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2SO4["K2SO4"]
    CultureMech_002906 --> ing3_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing3_K2SO4 -.-> CHEBI_32036
    ing4_KNO3["KNO3"]
    CultureMech_002906 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_H3BO3["H3BO3"]
    CultureMech_002906 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002906 --> ing6_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing6_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing7_NaOH["NaOH"]
    CultureMech_002906 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_Na2SeO3["Na2SeO3"]
    CultureMech_002906 --> ing8_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing8_Na2SeO3 -.-> CHEBI_48843
    ing9_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002906 --> ing9_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing9_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002906 --> ing10_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002906 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_Sodium_benzoate["Sodium benzoate"]
    CultureMech_002906 --> ing12_Sodium_benzoate
    CHEBI_113455["CHEBI:113455"]
    ing12_Sodium_benzoate -.-> CHEBI_113455
    ing13_Vitamin_B12["Vitamin B12"]
    CultureMech_002906 --> ing13_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_Vitamin_B12 -.-> CHEBI_176843
    ing14_D_Biotin["D-Biotin"]
    CultureMech_002906 --> ing14_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_D_Biotin -.-> CHEBI_15956
    ing15_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_002906 --> ing15_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_D_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002906 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002906 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002906 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002906 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Na2_EDTA["Na2-EDTA"]
    CultureMech_002906 --> ing20_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing20_Na2_EDTA -.-> CHEBI_64734
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002906 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
1.58103 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.988142 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.494071 G_PER_L CHEBI:31206
ammonium chloride
4 K2SO4
2K.O4S 174.259 g/mol
0.0592885 G_PER_L CHEBI:32036
potassium sulfate
5 KNO3
K.NO3 101.102 g/mol
0.503953 G_PER_L CHEBI:63043
potassium nitrate
6 H3BO3
H3BO3 61.833 g/mol
0.06 G_PER_L CHEBI:33118
boric acid
7 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
8 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
9 Na2SeO3
2Na.O3Se 172.937 g/mol
0.002 G_PER_L CHEBI:48843
disodium selenite
10 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
11 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
20 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
5 G_PER_L CHEBI:86158
calcium chloride dihydrate
13 Sodium benzoate
C7H5O2.Na 144.105 g/mol
72 G_PER_L CHEBI:113455
sodium benzoate
14 Vitamin B12 0.04 G_PER_L CHEBI:176843
vitamin B12
15 D-Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
16 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.1 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
20 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
21 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
22 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate

Preparation

  1. Adjust pH to 7.2 - 7.4.
  2. Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.929515Z mediadive-import Imported from MediaDive Source: JCM, ID: J558
2026-01-28T19:48:15.476871Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:16.478986Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002906
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.036112+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MAGNETOSPIRILLUM MEDIUM (A)" to "magnetospirillum_medium_a" for consistent matching
2026-06-05T07:41:12.555631+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.065095+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.802828+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:41.660191+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint dcf86c84f3f01fe0c41b057e0b67d68eb84593fe08ed6ada8e16bb3041a7a3cb