clostridiisalibacter_paucivorans_medium

CultureMech:002921 bacterial COMPLEX LIQUID
Original name: CLOSTRIDIISALIBACTER PAUCIVORANS MEDIUM

Overview

External ontology
mediadive.medium:J573 (source link unavailable) — CLOSTRIDIISALIBACTER PAUCIVORANS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=573

Composition

flowchart LR
    CultureMech_002921["clostridiisalibacter_paucivorans_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002921 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002921 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002921 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002921 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_002921 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2["CaCl2"]
    CultureMech_002921 --> ing5_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing5_CaCl2 -.-> CHEBI_3312
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002921 --> ing6_Yeast_extract
    ing7_Resazurin["Resazurin"]
    CultureMech_002921 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002921 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002921 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002921 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002921 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002921 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002921 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002921 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002921 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002921 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002921 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002921 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002921 --> ing19_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing19_AlK_SO4_2 -.-> CHEBI_86463
    ing20_H3BO3["H3BO3"]
    CultureMech_002921 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002921 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.329308 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.329308 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.09769 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
44.9078 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 43.9078, 1.0]
5 KCl
Cl.K 74.551 g/mol
0.109769 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2
Ca.2Cl 110.984 g/mol
0.109769 G_PER_L CHEBI:3312
calcium dichloride
7 Yeast extract 2.19539 G_PER_L
8 Resazurin
C12H7NO4 229.191 g/mol
0.00109769 G_PER_L CHEBI:8806
Resazurin
9 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
10 NaHCO3
CHO3.Na 84.006 g/mol
0.3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.1 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients and adjust pH to 7.0. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
  2. To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml):
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.971002Z mediadive-import Imported from MediaDive Source: JCM, ID: J573
2026-03-13T03:09:22.754313Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:16.578758Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002921
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.147574+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIISALIBACTER PAUCIVORANS MEDIUM" to "clostridiisalibacter_paucivorans_medium" for consistent matching
2026-06-05T07:40:53.670978+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.603475+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:27.781879+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ca7bf3195f763b3f08d9c144f02d9f519cc081a03415d558dc8504580ad7c244