bacillus_aa_medium

CultureMech:002927 bacterial COMPLEX LIQUID
Original name: BACILLUS AA MEDIUM

Overview

External ontology
mediadive.medium:J579 (source link unavailable) — BACILLUS AA MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=579

Composition

flowchart LR
    CultureMech_002927["bacillus_aa_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002927 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002927 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002927 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002927 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_002927 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002927 --> ing5_Yeast_extract
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002927 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002927 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_CaCl2["CaCl2"]
    CultureMech_002927 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002927 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002927 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002927 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002927 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002927 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002927 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002927 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002927 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002927 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002927 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002927 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002927 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.296736 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.296736 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.98912 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
1.98912 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.98912, 1.0]
5 KCl
Cl.K 74.551 g/mol
0.098912 G_PER_L CHEBI:32588
potassium chloride
6 Yeast extract 0.98912 G_PER_L
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.2 G_PER_L CHEBI:32954
sodium acetate
8 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 CaCl2
Ca.2Cl 110.984 g/mol
10 G_PER_L CHEBI:3312
calcium dichloride
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
50 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients and adjust pH to 7.2. Distribute the solution into tubes (e.g., 10 ml in the Hungate tubes) under a N2-CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave.
  2. To complete medium, aseptically add the following solutions to the medium (per 10 ml):
  3. Autoclave under a N2 atmosphere.
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.992286Z mediadive-import Imported from MediaDive Source: JCM, ID: J579
2026-03-13T03:08:41.922190Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:16.626310Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002927
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.199732+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS AA MEDIUM" to "bacillus_aa_medium" for consistent matching
2026-06-05T07:40:50.213864+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.693562+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:24.552286+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9a3e01687a3001dabd707d4056de8d81c867c167978ae1a0f94e29716ed892f2