rhodobacter_sphaeroides_medium

Overview

External ontology

mediadive.medium:J580 (source link unavailable) — RHODOBACTER SPHAEROIDES MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=580

Composition

flowchart LR
    CultureMech_002928["rhodobacter_sphaeroides_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002928 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002928 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NaCl["NaCl"]
    CultureMech_002928 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002928 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002928 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sorbitol["Sorbitol"]
    CultureMech_002928 --> ing5_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing5_Sorbitol -.-> CHEBI_30911
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002928 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002928 --> ing7_Yeast_extract
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_002928 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_HCl["HCl"]
    CultureMech_002928 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002928 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002928 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002928 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002928 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002928 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002928 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002928 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

Preparation

1. Adjust pH to 7.0.
2. Add the following component after autoclaving:

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Rhodobacter sphaeroides

  Term

  Id

  NCBITaxon:1063

  Label

  Cereibacter sphaeroides

  Growth metrics

  • Doubling time minutes

    1080.0

    Evidence

    • Reference

      PMID:19826864

      Supports

      SUPPORT

      Snippet

      Rhodobacter sphaeroides OU5 grows phototrophically with generation doubling time of 18 h on L-phenylalanine when used as sole source of nitrogen.

      Explanation

      Auto-applied from literature query: backfill PMID:19826864

    Is max attainment

    False

    Growth mode

    BATCH

    Nutrient overrides

    • Role

      NITROGEN_SOURCE

      Source

      L-phenylalanine

      Is sole source

      True

      Ontology id

      CHEBI:17295

Recipe variants

• Name

  phototrophic_l_phenylalanine_sole_nitrogen_ou5

  Description

  Phototrophic Rhodobacter sphaeroides OU5 growth using L-phenylalanine as the sole nitrogen source.

  Modifications

  • Use Rhodobacter sphaeroides strain OU5.
  • Use L-phenylalanine as the sole nitrogen source.
  • Culture phototrophically.

  Purpose

  Capture organism- and nitrogen-source-specific growth evidence under the parent Rhodobacter sphaeroides Medium record.

  Evidence

  • Reference

    PMID:19826864

    Supports

    SUPPORT

    Snippet

    Rhodobacter sphaeroides OU5 grows phototrophically with generation doubling time of 18 h on L-phenylalanine when used as sole source of nitrogen.

    Explanation

    Literature evidence supports a phototrophic L-phenylalanine sole-nitrogen variant for Rhodobacter sphaeroides OU5.

Provenance

Merged from 1 source record.

• rhodobacter_sphaeroides_medium (CultureMech:002928)