Overview
- External ontology
- mediadive.medium:J593 (source link unavailable) — MODIFIED 9K MEDIUM FOR FERROPLASMA AEOLICUM
- Applications
-
- Microbial cultivation
- pH
- 1.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=593
Composition
flowchart LR
CultureMech_002941["modified_9k_medium_for_ferroplasma_aeolicum"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002941 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002941 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_KCl["KCl"]
CultureMech_002941 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_K2HPO4["K2HPO4"]
CultureMech_002941 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002941 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Yeast_extract["Yeast extract"]
CultureMech_002941 --> ing5_Yeast_extract
ing6_HCl["HCl"]
CultureMech_002941 --> ing6_HCl
CHEBI_17883["CHEBI:17883"]
ing6_HCl -.-> CHEBI_17883
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002941 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_ZnCl2["ZnCl2"]
CultureMech_002941 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002941 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_002941 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002941 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002941 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002941 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002941 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.399202 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.199601 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.0998004 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.0998004 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
24.9501 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 | Yeast extract | 0.199601 G_PER_L | ||
| 7 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 1.6 - 1.7 with 10 N H2SO4 and autoclave. After cooling, add filter-sterilized K2S4O6 solution to give a final concentration of 5 mM.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_9k_medium_for_ferroplasma_aeolicum (CultureMech:002941)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.030068Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J593 |
| 2026-03-13T06:19:16.707650Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002941 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.298581+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED 9K MEDIUM FOR FERROPLASMA AEOLICUM" to "modified_9k_medium_for_ferroplasma_aeolicum" for consistent matching |
| 2026-06-05T07:41:36.094331+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.486076+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.734782+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5608273845c2048500a490911e93c8f8655891d021890872d53bc2fa41e7b73a |