Overview
- External ontology
- mediadive.medium:J602 (source link unavailable) — DESULFOVIBRIO MAGNUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=602
Composition
flowchart LR
CultureMech_002950["desulfovibrio_magnus_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_002950 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_002950 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_002950 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_002950 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na2SO4["Na2SO4"]
CultureMech_002950 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_Sodium_lactate["Sodium lactate"]
CultureMech_002950 --> ing5_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing5_Sodium_lactate -.-> CHEBI_75228
ing6_HCl["HCl"]
CultureMech_002950 --> ing6_HCl
CHEBI_17883["CHEBI:17883"]
ing6_HCl -.-> CHEBI_17883
ing7_H3BO3["H3BO3"]
CultureMech_002950 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002950 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002950 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002950 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002950 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_ZnCl2["ZnCl2"]
CultureMech_002950 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_NaOH["NaOH"]
CultureMech_002950 --> ing13_NaOH
CHEBI_32145["CHEBI:32145"]
ing13_NaOH -.-> CHEBI_32145
ing14_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002950 --> ing14_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing14_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing15_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002950 --> ing15_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing15_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002950 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002950 --> ing17_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
ing18_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002950 --> ing18_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing18_MgCl2_x_6_H2O -.-> CHEBI_86345
ing19_NaHCO3["NaHCO3"]
CultureMech_002950 --> ing19_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing19_NaHCO3 -.-> CHEBI_32139
ing20_Biotin["Biotin"]
CultureMech_002950 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_002950 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002950 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002950 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002950 --> ing24_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_DL_Calcium_pantothenate -.-> CHEBI_31345
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002950 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_002950 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_Riboflavin["Riboflavin"]
CultureMech_002950 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.587583 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.454545 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.332594 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.332594 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.57428 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
2.48337 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 7 |
HCl
HCl 36.461 g/mol
|
10 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.0611881 G_PER_L |
CHEBI:33118
boric acid
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0606436 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.934158 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0638614 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0127327 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0670297 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 15 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0173 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 16 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0294 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0205 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 19 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 20 |
NaHCO3
CHO3.Na 84.006 g/mol
|
40 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.1 G_PER_L |
CHEBI:17015
riboflavin
|
Preparation
- Mix components and autoclave under a N2 gas atmosphere.
- Mix components and autoclave under a N2 atmosphere.
- Mix components, filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.
- To complete the medium, combine Solutions A, B, C and 1 ml Vitamin solution (see below) aseptically under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into sterilized culture vessels under the same gas mixture and seal with butyl rubber stoppers. Then, add 0.01 volume of 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Check the final pH to be 7.0 - 7.2. Pressurize the inoculated vessels to 70 kPa N2-CO2 (4:1, v/v) overpressure.
- Filter-sterilize and store under a N2 atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfovibrio_magnus_medium (CultureMech:002950)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.059417Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J602 |
| 2026-03-13T06:19:16.777420Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002950 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.377980+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIBRIO MAGNUS MEDIUM" to "desulfovibrio_magnus_medium" for consistent matching |
| 2026-06-05T07:40:57.847439+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.052084+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:32.251189+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 63453b2901147eacee33cf703402baab587137f4f8573c78037c223c290734e1 |