jcm_medium_no_611

CultureMech:002959 bacterial COMPLEX SOLID_AGAR
Original name: JCM MEDIUM No. 611

Overview

External ontology
mediadive.medium:J611 (source link unavailable) — JCM MEDIUM No. 611
Applications
  • Microbial cultivation
pH
9.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=611

Composition

flowchart LR
    CultureMech_002959["jcm_medium_no_611"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002959 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002959 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002959 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002959 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_KCl["KCl"]
    CultureMech_002959 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002959 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002959 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_NaCl["NaCl"]
    CultureMech_002959 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar["Agar"]
    CultureMech_002959 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Casamino acids 5.55556 G_PER_L
2 Yeast extract 5.55556 G_PER_L
3 Na glutamate
C5H8NO4.Na 169.112 g/mol
1.11111 G_PER_L CHEBI:64220
monosodium glutamate
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3.33333 G_PER_L CHEBI:53258
sodium citrate
5 KCl
Cl.K 74.551 g/mol
2.22222 G_PER_L CHEBI:32588
potassium chloride
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.222222 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.04 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
8 NaCl
Cl.Na 58.443 g/mol
111.111 G_PER_L CHEBI:26710
sodium chloride
9 Agar 22.2222 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.3.
  2. After autoclaving, aseptically add 100 ml of 10% Na2CO3 solution to the medium. Check final pH to be about 9.5.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.086546Z mediadive-import Imported from MediaDive Source: JCM, ID: J611
2026-01-28T19:48:28.394568Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:16.828268Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002959
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.436890+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 611" to "jcm_medium_no_611" for consistent matching
2026-06-05T07:41:10.191942+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.239712+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:05:39.394884+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 84b0b3ffa66a70775f8bafadd3fb3ffc0335d5c5f232895ff411317dbe7d79bd