Overview
- External ontology
- mediadive.medium:J623 (source link unavailable) — JCM MEDIUM No. 623
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=623
Composition
flowchart LR
CultureMech_002969["jcm_medium_no_623"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002969 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002969 --> ing1_Tryptone
ing2_Agar["Agar"]
CultureMech_002969 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_NaCl["NaCl"]
CultureMech_002969 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002969 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_002969 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002969 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 20 G_PER_L | ||
| 2 | Tryptone | 5 G_PER_L | ||
| 3 | Agar | 40 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
10 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Adjust pH to 7.4.
- Separately autoclave Solutions A and B and mix them aseptically. Adjust pH to 7.4, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_623 (CultureMech:002969)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.119015Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J623 |
| 2026-03-13T06:19:16.886590Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002969 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.502198+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 623" to "jcm_medium_no_623" for consistent matching |
| 2026-06-05T07:41:10.197416+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:39.402025+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8bb966e0e0d8d1574b5af3818bf1f6d57863941f1d713d4157793410ca8fd016 |