Overview
- External ontology
- mediadive.medium:J626 (source link unavailable) — FERVIDOBACTERIUM TIANCHISUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=626
Composition
flowchart LR
CultureMech_002972["fervidobacterium_tianchisum_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002972 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_002972 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002972 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002972 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002972 --> ing4_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
ing5_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002972 --> ing5_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing5_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002972 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002972 --> ing7_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002972 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002972 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_Glucose["Glucose"]
CultureMech_002972 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_Tryptone["Tryptone"]
CultureMech_002972 --> ing11_Tryptone
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002972 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Sulfur["Sulfur"]
CultureMech_002972 --> ing13_Sulfur
CHEBI_26833["CHEBI:26833"]
ing13_Sulfur -.-> CHEBI_26833
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 6 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
5e-05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1e-05 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 12 | Tryptone | 4 G_PER_L | ||
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Sulfur
S 32.067 g/mol
|
5 G_PER_L |
CHEBI:26833
sulfur atom
|
Preparation
- Mix ingredients except glucose, sulfur and Na2S x 9H2O, and autoclave under a N2 atmosphere. Separately autoclave glucose as a 20% solution and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add glucose and sulfur to the medium. Prior to inoculation, add sterile Na2S x 9H2O solution and adjust pH to 7.5 with sterile 1 N H2SO4.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- fervidobacterium_tianchisum_medium (CultureMech:002972)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.125456Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J626 |
| 2026-03-13T06:19:16.901425Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002972 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.518332+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FERVIDOBACTERIUM TIANCHISUM MEDIUM" to "fervidobacterium_tianchisum_medium" for consistent matching |
| 2026-06-05T07:41:00.338308+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.859562+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:34.358384+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c3a8dacb7200004706e77a2a3566bc58f22d73aa97b68e87493e800e864e241c |