Overview
- External ontology
- mediadive.medium:J654 (source link unavailable) — POSTGATE J. R'S MEDIUM C
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=654
Composition
flowchart LR
CultureMech_003000["postgate_j_rs_medium_c"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003000 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_003000 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Na2SO4["Na2SO4"]
CultureMech_003000 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003000 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003000 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Yeast_extract["Yeast extract"]
CultureMech_003000 --> ing5_Yeast_extract
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003000 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Sodium_citrate["Sodium citrate"]
CultureMech_003000 --> ing7_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing7_Sodium_citrate -.-> CHEBI_53258
ing8_Sodium_lactate["Sodium lactate"]
CultureMech_003000 --> ing8_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing8_Sodium_lactate -.-> CHEBI_75228
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4.5 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.06 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | Yeast extract | 1 G_PER_L | ||
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 9 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
100 G_PER_L |
CHEBI:75228
sodium lactate
|
Preparation
- Mix components thoroughly and autoclave. Then add the following solution:
- Cultivate under a N2 atmosphere.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- postgate_j_rs_medium_c (CultureMech:003000)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.198638Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J654 |
| 2026-03-13T06:19:17.068887Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003000 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.711034+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "POSTGATE J. R'S MEDIUM C" to "postgate_j_rs_medium_c" for consistent matching |
| 2026-06-05T07:41:42.366485+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.360671+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.373011+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f76663b62befa9d7d4e37c5c33c909bf071bf8ce1deeef302088575b7b097d30 |