magnetospirillum_gryphiswaldense_medium

Overview

External ontology

mediadive.medium:J660 (source link unavailable) — MAGNETOSPIRILLUM GRYPHISWALDENSE MEDIUM

Applications

• Microbial cultivation

pH

6.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=660

Composition

flowchart LR
    CultureMech_003006["magnetospirillum_gryphiswaldense_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003006 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003006 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_003006 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003006 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_003006 --> ing4_Yeast_extract
    ing5_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_003006 --> ing5_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing5_Sodium_thioglycolate -.-> CHEBI_86481
    ing6_Ferric_citrate["Ferric citrate"]
    CultureMech_003006 --> ing6_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing6_Ferric_citrate -.-> CHEBI_144421

Ingredients (7)

Preparation

1. Adjust pH to 6.8.
2. Comment: For strain JCM 21280, good growth can be obtained in semisolid medium (1.0 g/L agar).

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  MAGNETOSPIRILLUM GRYPHISWALDENSE

  Term

  Id

  NCBITaxon:55518

  Label

  Magnetospirillum gryphiswaldense

• Preferred term

  Magnetospirillum gryphiswaldense

  Term

  Id

  NCBITaxon:55518

  Label

  Magnetospirillum gryphiswaldense

  Strain modifications

  • Modification type

    KNOCKOUT

    Target

    magnetosome operon

    Description

    magnetosome-deficient strain B17316

    Ontology id

    NCIT:C120956

  Growth metrics

  • Growth rate per hour

    0.062

    Evidence

    • Reference

      PMID:37088211

      Supports

      SUPPORT

      Snippet

      The addition of 10 mg L-1 Cr(VI) significantly inhibited cell growth, but the magnetosome-deficient strain, B17316, showed an average specific growth rate of 0.062 h-1 at the same dosage.

      Explanation

      Auto-applied from literature query: backfill PMID:37088211

    Is max attainment

    False

    Growth mode

    BATCH

    Perturbations

    • Perturbation type

      CHEMICAL_STRESS

      Descriptor

      Cr(VI) at 10 mg/L

      Target

      chromium hexavalent

      Level

      10.0

      Level unit

      mg/L

      Ontology id

      CHEBI:53456

Recipe variants

• Name

  crvi_stress_magnetosome_deficient_b17316

  Description

  Cr(VI) stress condition for magnetosome-deficient Magnetospirillum gryphiswaldense strain B17316.

  Modifications

  • Use magnetosome-deficient strain B17316 derived from M. gryphiswaldense MSR-1.
  • Add Cr(VI) at 10 mg/L.

  Purpose

  Capture strain-modification and chromium-stress growth evidence under the parent Magnetospirillum gryphiswaldense Medium record.

  Evidence

  • Reference

    PMID:37088211

    Supports

    SUPPORT

    Snippet

    The addition of 10 mg L-1 Cr(VI) significantly inhibited cell growth, but the magnetosome-deficient strain, B17316, showed an average specific growth rate of 0.062 h-1 at the same dosage.

    Explanation

    Literature evidence supports a Cr(VI)-stress variant for magnetosome-deficient Magnetospirillum gryphiswaldense strain B17316.

Provenance

Merged from 1 source record.

• magnetospirillum_gryphiswaldense_medium (CultureMech:003006)