Overview
- External ontology
- mediadive.medium:J661 (source link unavailable) — MODIFIED AM5 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=661
Composition
flowchart LR
CultureMech_003007["modified_am5_medium"]
ing0_NaCl["NaCl"]
CultureMech_003007 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_003007 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003007 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003007 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_003007 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_003007 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Na2SO4["Na2SO4"]
CultureMech_003007 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Resazurin["Resazurin"]
CultureMech_003007 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_NaHCO3["NaHCO3"]
CultureMech_003007 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
CultureMech_003007 --> ing9_Trace_vitamins__see_Medium_No__197_
ing10_Fatty_acid_mixture__see_Medium_No__266_["Fatty acid mixture (see Medium No. 266)"]
CultureMech_003007 --> ing10_Fatty_acid_mixture__see_Medium_No__266_
CHEBI_35366["CHEBI:35366"]
ing10_Fatty_acid_mixture__see_Medium_No__266_ -.-> CHEBI_35366
ing11_Yeast_extract["Yeast extract"]
CultureMech_003007 --> ing11_Yeast_extract
ing12_Casamino_acids["Casamino acids"]
CultureMech_003007 --> ing12_Casamino_acids
ing13_Glucose["Glucose"]
CultureMech_003007 --> ing13_Glucose
CHEBI_17234["CHEBI:17234"]
ing13_Glucose -.-> CHEBI_17234
ing14_Dithiothreitol["Dithiothreitol"]
CultureMech_003007 --> ing14_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing14_Dithiothreitol -.-> CHEBI_18320
ing15_ZnCl2["ZnCl2"]
CultureMech_003007 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003007 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_003007 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003007 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003007 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003007 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003007 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_HCl["HCl"]
CultureMech_003007 --> ing22_HCl
CHEBI_17883["CHEBI:17883"]
ing22_HCl -.-> CHEBI_17883
ing23_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003007 --> ing23_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing23_FeCl2_x_4_H2O -.-> CHEBI_86249
ing24_NaOH["NaOH"]
CultureMech_003007 --> ing24_NaOH
CHEBI_32145["CHEBI:32145"]
ing24_NaOH -.-> CHEBI_32145
ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003007 --> ing25_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003007 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.15 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
40 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 | Trace vitamins (see Medium No. 197) | 10 G_PER_L | ||
| 11 |
Fatty acid mixture (see Medium No. 266)
CHO2R 45.017 g/mol
|
1 G_PER_L |
CHEBI:35366
fatty acid
|
|
| 12 | Yeast extract | 10 G_PER_L | ||
| 13 | Casamino acids | 10 G_PER_L | ||
| 14 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 15 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
10 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 24 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 25 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 26 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Solutions
Vitamin B12 solution (see Medium No. 403)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_am5_medium (CultureMech:003007)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.214456Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J661 |
| 2026-01-28T19:48:15.046186Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:25.033450+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:17.111737Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003007 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.760733+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED AM5 MEDIUM" to "modified_am5_medium" for consistent matching |
| 2026-05-16T09:04:25.008364+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:36.133688+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.679412+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:08.500324+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.921707+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 421a5fe802cd8f281da4f143ae001fda86e6f848435c60f307c68acee990f7df |