magnetospirillum_medium_b

CultureMech:003013 bacterial DEFINED LIQUID
Original name: MAGNETOSPIRILLUM MEDIUM (B)

Overview

External ontology
mediadive.medium:J669 (source link unavailable) — MAGNETOSPIRILLUM MEDIUM (B)
Applications
  • Microbial cultivation
pH
6.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=669

Composition

flowchart LR
    CultureMech_003013["magnetospirillum_medium_b"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003013 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaNO3["NaNO3"]
    CultureMech_003013 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_003013 --> ing2_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing2_Sodium_thioglycolate -.-> CHEBI_86481
    ing3_Sodium_tartrate["Sodium tartrate"]
    CultureMech_003013 --> ing3_Sodium_tartrate
    CHEBI_63017["CHEBI:63017"]
    ing3_Sodium_tartrate -.-> CHEBI_63017
    ing4_Sodium_succinate["Sodium succinate"]
    CultureMech_003013 --> ing4_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing4_Sodium_succinate -.-> CHEBI_63675
    ing5_Sodium_acetate["Sodium acetate"]
    CultureMech_003013 --> ing5_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Sodium_acetate -.-> CHEBI_32954
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003013 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Quinic_acid["Quinic acid"]
    CultureMech_003013 --> ing7_Quinic_acid
    CHEBI_26493["CHEBI:26493"]
    ing7_Quinic_acid -.-> CHEBI_26493
    ing8_Biotin["Biotin"]
    CultureMech_003013 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Folic_acid["Folic acid"]
    CultureMech_003013 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003013 --> ing10_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003013 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Riboflavin["Riboflavin"]
    CultureMech_003013 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003013 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003013 --> ing14_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_pantothenate -.-> CHEBI_31345
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_003013 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003013 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Lipoic_acid["Lipoic acid"]
    CultureMech_003013 --> ing17_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing17_Lipoic_acid -.-> CHEBI_16494
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003013 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003013 --> ing19_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003013 --> ing20_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003013 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003013 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.276407 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NaNO3
NO3.Na 84.994 g/mol
0.11846 G_PER_L CHEBI:63005
sodium nitrate
3 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
0.0493583 G_PER_L CHEBI:86481
sodium thioglycolate
4 Sodium tartrate
C4H4O6.2Na 194.05 g/mol
0.365252 G_PER_L CHEBI:63017
sodium L-tartrate
5 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.365252 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
6 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.0493583 G_PER_L CHEBI:32954
sodium acetate
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
4.5 G_PER_L CHEBI:86254
iron trichloride hexahydrate
8 Quinic acid 1.9 G_PER_L CHEBI:26493
quinic acid
9 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
13 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
15 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
16 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
21 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
22 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.099 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.013 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 6.75 with NaOH.
  2. Dissolve and autoclave at 121C for 15 min.
  3. Comment: For strain JCM 21281, good growth can be obtained in semisolid medium (1.0 g/L agar).
  4. Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.233142Z mediadive-import Imported from MediaDive Source: JCM, ID: J669
2026-03-13T06:19:17.143788Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003013
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.796478+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MAGNETOSPIRILLUM MEDIUM (B)" to "magnetospirillum_medium_b" for consistent matching
2026-06-05T07:41:12.568668+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.807836+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:41.676262+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2dfc58b71433531d4e24ead6d0864428d64c757bda0202cc4d943df065ee85d0