Overview
- External ontology
- mediadive.medium:J674 (source link unavailable) — PYG MEDIUM (F)
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=674
Composition
flowchart LR
CultureMech_003019["pyg_medium_f"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_003019 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003019 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003019 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_003019 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Na2CO3["Na2CO3"]
CultureMech_003019 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003019 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_K2HPO4["K2HPO4"]
CultureMech_003019 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_KH2PO4["KH2PO4"]
CultureMech_003019 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_003019 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_NaCl["NaCl"]
CultureMech_003019 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003019 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003019 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 9.96016 G_PER_L | ||
| 2 | Yeast extract | 4.98008 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
9.96016 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.298805 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Adjust pH to 7.3.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyg_medium_f (CultureMech:003019)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.256210Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J674 |
| 2026-03-13T06:19:17.211279Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003019 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.868494+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG MEDIUM (F)" to "pyg_medium_f" for consistent matching |
| 2026-06-05T07:41:43.068491+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.589359+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.918073+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint baaaef5a3cf234cdfc4a9f5a508bc9ffdd7f8e0ec19615de58736875a53a140d |