halanaerobaculum_tunisiense_medium

Overview

External ontology

mediadive.medium:J678 (source link unavailable) — HALANAEROBACULUM TUNISIENSE MEDIUM

Applications

• Microbial cultivation

pH

8.3

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=678

Composition

flowchart LR
    CultureMech_003023["halanaerobaculum_tunisiense_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003023 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003023 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003023 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003023 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003023 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003023 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KNO3["KNO3"]
    CultureMech_003023 --> ing6_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing6_KNO3 -.-> CHEBI_63043
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_003023 --> ing7_Yeast_extract
    ing8_NaHCO3["NaHCO3"]
    CultureMech_003023 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003023 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003023 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003023 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003023 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003023 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003023 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003023 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003023 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003023 --> ing17_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing17_AlK_SO4_2 -.-> CHEBI_86463
    ing18_H3BO3["H3BO3"]
    CultureMech_003023 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003023 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

Preparation

1. Mix components and adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N2 gas stream. Then, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• halanaerobaculum_tunisiense_medium (CultureMech:003023)