Overview
- External ontology
- mediadive.medium:J682 (source link unavailable) — METHANOFOLLIS ETHANOLICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=682
Composition
flowchart LR
CultureMech_003028["methanofollis_ethanolicus_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003028 --> ing0_Yeast_extract
ing1_KH2PO4["KH2PO4"]
CultureMech_003028 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003028 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003028 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_003028 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_NaHCO3["NaHCO3"]
CultureMech_003028 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Resazurin["Resazurin"]
CultureMech_003028 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Ethanol["Ethanol"]
CultureMech_003028 --> ing7_Ethanol
CHEBI_16236["CHEBI:16236"]
ing7_Ethanol -.-> CHEBI_16236
ing8_Biotin["Biotin"]
CultureMech_003028 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Folic_acid["Folic acid"]
CultureMech_003028 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003028 --> ing10_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing11_Thiamine_HCl["Thiamine HCl"]
CultureMech_003028 --> ing11_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing11_Thiamine_HCl -.-> CHEBI_49105
ing12_Riboflavin["Riboflavin"]
CultureMech_003028 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_003028 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_003028 --> ing14_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_DL_Calcium_pantothenate -.-> CHEBI_31345
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_003028 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003028 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_Lipoic_acid["Lipoic acid"]
CultureMech_003028 --> ing17_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing17_Lipoic_acid -.-> CHEBI_16494
ing18_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003028 --> ing18_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing18_FeCl3_x_6_H2O -.-> CHEBI_86254
ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003028 --> ing19_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003028 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
ing21_ZnCl2["ZnCl2"]
CultureMech_003028 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003028 --> ing22_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
ing23_AlCl3["AlCl3"]
CultureMech_003028 --> ing23_AlCl3
CHEBI_30114["CHEBI:30114"]
ing23_AlCl3 -.-> CHEBI_30114
ing24_H3BO3["H3BO3"]
CultureMech_003028 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003028 --> ing25_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003028 --> ing26_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
ing27_Na2SeO3["Na2SeO3"]
CultureMech_003028 --> ing27_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing27_Na2SeO3 -.-> CHEBI_48843
ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003028 --> ing28_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.0997009 G_PER_L | ||
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.139581 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199402 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.149551 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.538385 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.49252 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000997009 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Ethanol
C2H6O 46.069 g/mol
|
0.46 G_PER_L |
CHEBI:16236
ethanol
|
|
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0049 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0088 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0041 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 12 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0067 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0075 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.0025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0095 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 16 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.0041 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 19 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.27 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 20 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.13 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.14 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 23 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.001 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 24 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 27 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 28 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.002 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 29 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.003 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Solution A:
- Mix components, except NaHCO3, and adjust pH to 6.5. Bring to a boil for 5-10 sec., then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense 0.9 volume of the medium into culture vessels (e.g., 18 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight.
- Solution B:
- Adjust pH to 7.0 and filter-sterilize. Keep the solution under a N2 atmosphere.
- To complete the medium, add 0.1 volume of solution B to the culture vessels containing solution A. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O and Na2S x 9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere). Incubate with a 10% inoculum.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanofollis_ethanolicus_medium (CultureMech:003028)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.282661Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J682 |
| 2026-03-13T06:19:17.284023Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003028 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.954593+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOFOLLIS ETHANOLICUS MEDIUM" to "methanofollis_ethanolicus_medium" for consistent matching |
| 2026-06-05T07:41:31.416483+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.935210+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.332787+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a022ebcd9098a274c206217475566bc1e3248bbeae1730b3856ef4c09e6b9d5b |