mb_medium_with_methanol

CultureMech:003032 bacterial COMPLEX LIQUID
Original name: MB MEDIUM WITH METHANOL

Overview

External ontology
mediadive.medium:J687 (source link unavailable) — MB MEDIUM WITH METHANOL
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=687

Composition

flowchart LR
    CultureMech_003032["mb_medium_with_methanol"]
    ing0_NaCl["NaCl"]
    CultureMech_003032 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003032 --> ing1_Yeast_extract
    ing2_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003032 --> ing2_Trypticase_peptone
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003032 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003032 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_003032 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003032 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_K2HPO4["K2HPO4"]
    CultureMech_003032 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_Resazurin["Resazurin"]
    CultureMech_003032 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003032 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003032 --> ing10_Trace_vitamins__see_Medium_No__197_
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003032 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003032 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Methanol["Methanol"]
    CultureMech_003032 --> ing13_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing13_Methanol -.-> CHEBI_17790
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003032 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003032 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003032 --> ing16_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003032 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003032 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003032 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003032 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003032 --> ing21_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing21_AlK_SO4_2 -.-> CHEBI_86463
    ing22_H3BO3["H3BO3"]
    CultureMech_003032 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003032 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NaOH["NaOH"]
    CultureMech_003032 --> ing24_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing24_NaOH -.-> CHEBI_32145
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003032 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003032 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
10.99001 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 9.99001, 1.0]
2 Yeast extract 1.998 G_PER_L
3 Trypticase peptone 1.998 G_PER_L
4 NH4Cl
Cl.H4N 53.492 g/mol
0.999001 G_PER_L CHEBI:31206
ammonium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.999001 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.499501 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.49960000000000004 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.3996, 0.1]
8 K2HPO4
HO4P.2K 174.174 g/mol
0.3996 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 Resazurin
C12H7NO4 229.191 g/mol
0.0004995 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
3.996 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Trace vitamins (see Medium No. 197) 10 G_PER_L
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Methanol
CH4O 32.042 g/mol
2.28 G_PER_L CHEBI:17790
methanol
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components except NaHCO3, bring to a boil for several seconds and cool down under a stream of N2-CO2 (80:20). Add NaHCO3, mix thoroughly and distribute the medium into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave. Finally, add per liter the following sterile solutions (autoclaved or *filter-sterilized):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Solutions

Methanol solution (see below)

Dose: 50 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.299292Z mediadive-import Imported from MediaDive Source: JCM, ID: J687
2026-03-09T08:04:22.577035+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:50.760398Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.315113Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003032
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.989429+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MB MEDIUM WITH METHANOL" to "mb_medium_with_methanol" for consistent matching
2026-05-16T09:03:40.573117+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:13.297486+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.020625+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.226487+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint fffc8864e314132358d935efe5ab99ba919aec21e2b239ff8c82de5bf9433d37