mjy_medium_for_pyrolinea_marinus

CultureMech:003046 bacterial COMPLEX LIQUID
Original name: MJY MEDIUM FOR PYROLINEA MARINUS

Overview

External ontology
mediadive.medium:J700 (source link unavailable) — MJY MEDIUM FOR PYROLINEA MARINUS
Applications
  • Microbial cultivation
pH
6.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=700

Composition

flowchart LR
    CultureMech_003046["mjy_medium_for_pyrolinea_marinus"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003046 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_NaNO3["NaNO3"]
    CultureMech_003046 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003046 --> ing2_Yeast_extract
    ing3_NaHCO3["NaHCO3"]
    CultureMech_003046 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003046 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_NaCl["NaCl"]
    CultureMech_003046 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_K2HPO4["K2HPO4"]
    CultureMech_003046 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003046 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003046 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003046 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_003046 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003046 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003046 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_003046 --> ing13_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing13_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.246305 G_PER_L CHEBI:31206
ammonium chloride
2 NaNO3
NO3.Na 84.994 g/mol
0.492611 G_PER_L CHEBI:63005
sodium nitrate
3 Yeast extract 0.985222 G_PER_L
4 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
6 NaCl
Cl.Na 58.443 g/mol
29.703 G_PER_L CHEBI:26710
sodium chloride
7 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.138614 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36634 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13861 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 KCl
Cl.K 74.551 g/mol
0.326733 G_PER_L CHEBI:32588
potassium chloride
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00049505 G_PER_L CHEBI:34887
nickel dichloride
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00049505 G_PER_L CHEBI:131361
disodium selenite pentahydrate
14 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.00990099 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate

Preparation

  1. Mix components thoroughly. Distribute the medium into culture vessels (e.g., 20 ml medium in 50 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (*filter-sterilized or autoclaved, and stored anaerobically) to the medium:
  2. Pressurize the culture vessels to 200 kPa N2-CO2 (4:1, v/v) . Check final pH to be 6.0-6.5.
  3. Comment: MJ(-N) synthetic seawater can be replaced with commercially available synthetic seawater such as Jamarin S (Jamarin Laboratory). Addition of rumen fluid clarified (10.0 ml/L, see Medium No. 266) may stimulate growth. For strain JCM 15506, incubate with a 5-10% inoculum.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.335714Z mediadive-import Imported from MediaDive Source: JCM, ID: J700
2026-01-28T19:48:42.842131Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:17.399166Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003046
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.084802+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MJY MEDIUM FOR PYROLINEA MARINUS" to "mjy_medium_for_pyrolinea_marinus" for consistent matching
2026-06-05T07:41:35.117881+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.189603+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:46.771054+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 65025182c4e676fe514ac6c05a0c7792c53b9f658f39aa11d0ff67ad8cb87701