Overview
- External ontology
- mediadive.medium:J702 (source link unavailable) — METHANOBACTERIUM MEDIUM (II)
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=702
Composition
flowchart LR
CultureMech_003048["methanobacterium_medium_ii"]
ing0_NH4Cl["NH4Cl"]
CultureMech_003048 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_003048 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_003048 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003048 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003048 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_003048 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaCl["NaCl"]
CultureMech_003048 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Sodium_acetate["Sodium acetate"]
CultureMech_003048 --> ing7_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Sodium_acetate -.-> CHEBI_32954
ing8_Yeast_extract["Yeast extract"]
CultureMech_003048 --> ing8_Yeast_extract
ing9_Resazurin["Resazurin"]
CultureMech_003048 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003048 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_NaHCO3["NaHCO3"]
CultureMech_003048 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003048 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003048 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003048 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003048 --> ing15_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing15_MnSO4_x_n_H2O -.-> CHEBI_86360
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003048 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003048 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003048 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003048 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003048 --> ing20_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing20_AlK_SO4_2 -.-> CHEBI_86463
ing21_H3BO3["H3BO3"]
CultureMech_003048 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003048 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Biotin["Biotin"]
CultureMech_003048 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_003048 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003048 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_003048 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_003048 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_003048 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003048 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_003048 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.947867 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.28436 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28436 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.189573 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1947867 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0947867, 0.1] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.0947867 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
1.56872 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.56872, 1.0] |
| 8 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.473934 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 | Yeast extract | 0.473934 G_PER_L | ||
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000473934 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.473934 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or *filter-sterilized):
- Check pH of the medium to be about 7.0. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanobacterium_medium_ii (CultureMech:003048)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.340750Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J702 |
| 2026-03-13T03:09:28.942709Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:17.414791Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003048 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.104857+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM MEDIUM (II)" to "methanobacterium_medium_ii" for consistent matching |
| 2026-06-05T07:41:30.731746+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.669981+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:29.549082+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d70fc1dd1d8c1ea1ae42eb00665bce6094e3323f3fc0a431524053966277b9e8 |