Overview
- External ontology
- mediadive.medium:J704 (source link unavailable) — ANOXYBACILLUS TUNISIENSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=704
Composition
flowchart LR
CultureMech_003050["anoxybacillus_tunisiense_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_003050 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003050 --> ing1_Yeast_extract
ing2_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003050 --> ing2_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing2_Nitrilotriacetic_acid -.-> CHEBI_44557
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003050 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_003050 --> ing4_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
ing5_KH2PO4["KH2PO4"]
CultureMech_003050 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003050 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003050 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003050 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003050 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_ZnCl2["ZnCl2"]
CultureMech_003050 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003050 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003050 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_003050 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003050 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003050 --> ing15_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing15_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing16_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003050 --> ing16_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing16_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 2.5 G_PER_L | ||
| 2 | Yeast extract | 2.5 G_PER_L | ||
| 3 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
14.120000000000001 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
[Merged 2 duplicates: 1.32, 12.8] |
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.4 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5.44 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
21.4 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.3 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.2 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.02 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.94 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 17 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
2.7 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Adjust pH to 8.0.
- Adjust pH to 7.2.
- Adjust pH to 6.0 - 6.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- anoxybacillus_tunisiense_medium (CultureMech:003050)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.347144Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J704 |
| 2026-01-28T19:48:38.084217Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:35.586328Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.428362Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003050 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.121883+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANOXYBACILLUS TUNISIENSE MEDIUM" to "anoxybacillus_tunisiense_medium" for consistent matching |
| 2026-06-05T07:40:49.316058+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.388273+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:23.751606+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3d9581d24ca554906ada5facaad1e65c7dc19e788a80efa138fefcc101594a39 |