Overview
- External ontology
- mediadive.medium:J705 (source link unavailable) — SELENOMONAS WG MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=705
Composition
flowchart LR
CultureMech_003051["selenomonas_wg_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003051 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003051 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_003051 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_NaCl["NaCl"]
CultureMech_003051 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003051 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003051 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Tryptone["Tryptone"]
CultureMech_003051 --> ing6_Tryptone
ing7_Yeast_extract["Yeast extract"]
CultureMech_003051 --> ing7_Yeast_extract
ing8_Glucose["Glucose"]
CultureMech_003051 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Resazurin["Resazurin"]
CultureMech_003051 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003051 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003051 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_Butyric_acid["Butyric acid"]
CultureMech_003051 --> ing12_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing12_Butyric_acid -.-> CHEBI_30772
ing13_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_003051 --> ing13_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing13_iso_Valeric_acid -.-> CHEBI_28484
ing14_n_Valeric_acid["n-Valeric acid"]
CultureMech_003051 --> ing14_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing14_n_Valeric_acid -.-> CHEBI_17418
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.490196 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.490196 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.980392 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.980392 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0980392 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0980392 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Tryptone | 4.90196 G_PER_L | ||
| 8 | Yeast extract | 1.96078 G_PER_L | ||
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1.96078 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
Butyric acid
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 14 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 15 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:17418
valeric acid
|
Preparation
- Mix components and adjust pH to 7.0. Bring to a boil for several seconds and cool down to room temperature under a stream of N2-CO2 (4:1, v/v). Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Prior to use, add per liter the following sterile solutions (autoclaved):
- Adjust pH to 6.7 - 6.8, and store in a tightly stoppered bottle at 4C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- selenomonas_wg_medium (CultureMech:003051)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:17.350440Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J705 |
| 2026-03-13T06:19:17.436041Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003051 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.132103+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SELENOMONAS WG MEDIUM" to "selenomonas_wg_medium" for consistent matching |
| 2026-06-05T07:41:45.690267+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.437587+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.663500+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 551ab349e0cfa2b03a9fc4f07b50d52e32534665c7ee622cd4fbf34d68118fd5 |