Overview
- External ontology
- mediadive.medium:J707 (source link unavailable) — LACTATE SULFATE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=707
Composition
flowchart LR
CultureMech_003053["lactate_sulfate_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003053 --> ing0_Yeast_extract
ing1_Na2SO4["Na2SO4"]
CultureMech_003053 --> ing1_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing1_Na2SO4 -.-> CHEBI_32149
ing2_L_Sodium_lactate["L-Sodium lactate"]
CultureMech_003053 --> ing2_L_Sodium_lactate
CHEBI_232798["CHEBI:232798"]
ing2_L_Sodium_lactate -.-> CHEBI_232798
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003053 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NH4Cl["NH4Cl"]
CultureMech_003053 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_003053 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003053 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Coenzyme_M["Coenzyme M"]
CultureMech_003053 --> ing7_Coenzyme_M
CHEBI_17905["CHEBI:17905"]
ing7_Coenzyme_M -.-> CHEBI_17905
ing8_HEPES["HEPES"]
CultureMech_003053 --> ing8_HEPES
CHEBI_46756["CHEBI:46756"]
ing8_HEPES -.-> CHEBI_46756
ing9_Resazurin["Resazurin"]
CultureMech_003053 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_NaHCO3["NaHCO3"]
CultureMech_003053 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003053 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Biotin["Biotin"]
CultureMech_003053 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_003053 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003053 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_003053 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Riboflavin["Riboflavin"]
CultureMech_003053 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_003053 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003053 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_003053 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003053 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Lipoic_acid["Lipoic acid"]
CultureMech_003053 --> ing21_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21_Lipoic_acid -.-> CHEBI_16494
ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003053 --> ing22_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
ing23_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003053 --> ing23_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4_x_7_H2O -.-> CHEBI_75836
ing24_H3BO3["H3BO3"]
CultureMech_003053 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003053 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003053 --> ing26_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003053 --> ing27_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
ing28_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003053 --> ing28_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing28_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003053 --> ing29_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.966184 G_PER_L | ||
| 2 |
Na2SO4
2Na.O4S 142.043 g/mol
|
6.8599 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 3 |
L-Sodium lactate
C3H5O3.Na 112.06 g/mol
|
6.71498 G_PER_L |
CHEBI:232798
sodium L-lactate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.90338 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.04348 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.36715 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0869565 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Coenzyme M
C2H6O3S2 142.201 g/mol
|
0.2 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 9 | HEPES | 5.75845 G_PER_L |
CHEBI:46756
HEPES
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000966184 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.050100000000000006 G_PER_L |
CHEBI:176843
vitamin B12
|
[Merged 2 duplicates: 0.0001, 0.05] |
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 23 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 24 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.42 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 27 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 28 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 29 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.21 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 30 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.2.
- Autoclave under a N2 gas atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized):
- Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- LACTATE SULFATE
Provenance
Merged from 1 source record.
- lactate_sulfate_medium (CultureMech:003053)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:17.356958Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J707 |
| 2026-02-20T07:49:55.000000Z | organism-data-backfill | Added curated organism data | Added organism: LACTATE SULFATE, culture_type: isolate |
| 2026-03-13T03:08:37.596025Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.457898Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003053 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.164963+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTATE SULFATE MEDIUM" to "lactate_sulfate_medium" for consistent matching |
| 2026-06-05T07:41:10.677095+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.457770+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.857988+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1190e91e524948b98db351d163586d1e74c45248448c2c5b635dc507a2581734 |